Formation of functional Tat translocases from heterologous components

Matthew G. Hicks, David Guymer, Grant Buchanan, David A. Widdick, Isabelle Caldelari, Ben C. Berks, Tracy Palmer

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    Abstract

    Background: The Tat pathway transports folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plants. In Eschericha coli, Tat transport requires the integral membrane proteins TatA, TatB and TatC. In this study we have tested the ability of tat genes from the eubacterial species Pseudomonas syringae, Streptomyces coelicolor and Aquifex aeolicus, to compensate for the absence of the cognate E. coli tat gene, and thus to form functional Tat translocases with E. coli Tat components.

    Results: All three subunits of the Tat system from the Gram positive organism Streptomyces coelicolor were able to form heterologous translocases with substantive Tat transport activity. However, only the TatA and TatB proteins of Pseudomonas syringae were able to functionally interact with the E. coli Tat system even though the two organisms are closely related. Of the Tat components from the phylogenetically distant hyperthermophillic bacterium Aquifex aeolicus only the TatA proteins showed any detectable level of heterologous functionality. The heterologously expressed TatA proteins of S. coelicolor and A. aeolicus were found exclusively in the membrane fraction.

    Conclusion: Our results show that of the three Tat proteins, TatA is most likely to show cross-species complementation. By contrast, TatB and TatC do not always show cross-complementation, probably because they must recognise heterologous signal peptides. Since heterologously-expressed S. coelicolor TatA protein was functional and found only in the membrane fraction, it suggests that soluble forms of Streptomyces TatA reported by others do not play a role in protein export.

    Original languageEnglish
    Article number64
    Number of pages12
    JournalBMC Microbiology
    Volume6
    DOIs
    Publication statusPublished - 2006

    Cite this

    Hicks, M. G., Guymer, D., Buchanan, G., Widdick, D. A., Caldelari, I., Berks, B. C., & Palmer, T. (2006). Formation of functional Tat translocases from heterologous components. BMC Microbiology, 6, [64]. https://doi.org/10.1186/1471-2180-6-64