Four zona pellucida glycoproteins are expressed in the human

L. Lefièvre; S. J. Conner; A. Salpekar; O. Olufowobi; P. Ashton; B. Pavlovic; W. Lenton; M. Afnan; I. A. Brewis; M. Monk; D. C. Hughes; C. L. R. Barratt

doi:10.1093/humrep/deh301

Four zona pellucida glycoproteins are expressed in the human

L. Lefièvre, S. J. Conner, A. Salpekar, O. Olufowobi, P. Ashton, B. Pavlovic, W. Lenton, M. Afnan, I. A. Brewis, M. Monk, D. C. Hughes, C. L. R. Barratt (Lead / Corresponding author)

Undergraduate Medicine

Research output: Contribution to journal › Article › peer-review

282 Citations (Scopus)

Abstract

Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.

Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).

Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.

Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model

Original language	English
Pages (from-to)	1580-1586
Number of pages	7
Journal	Human Reproduction
Volume	19
Issue number	7
DOIs	https://doi.org/10.1093/humrep/deh301
Publication status	Published - 1 Jul 2004

Keywords

Amino Acid Sequence
Animals
Base Sequence
Computational Biology/methods
Egg Proteins/genetics
Female
Gene Expression
Humans
Membrane Glycoproteins/genetics
Mice/genetics
Molecular Sequence Data
Oocytes/metabolism
Proteomics
Receptors, Cell Surface/genetics
Zona Pellucida/metabolism
Zona Pellucida Glycoproteins

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10.1093/humrep/deh301

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title = "Four zona pellucida glycoproteins are expressed in the human",

abstract = "Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Computational Biology/methods, Egg Proteins/genetics, Female, Gene Expression, Humans, Membrane Glycoproteins/genetics, Mice/genetics, Molecular Sequence Data, Oocytes/metabolism, Proteomics, Receptors, Cell Surface/genetics, Zona Pellucida/metabolism, Zona Pellucida Glycoproteins",

author = "L. Lefi{\`e}vre and Conner, {S. J.} and A. Salpekar and O. Olufowobi and P. Ashton and B. Pavlovic and W. Lenton and M. Afnan and Brewis, {I. A.} and M. Monk and Hughes, {D. C.} and Barratt, {C. L. R.}",

year = "2004",

month = jul,

day = "1",

doi = "10.1093/humrep/deh301",

language = "English",

volume = "19",

pages = "1580--1586",

journal = "Human Reproduction",

issn = "0268-1161",

publisher = "Oxford University Press",

number = "7",

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TY - JOUR

T1 - Four zona pellucida glycoproteins are expressed in the human

AU - Lefièvre, L.

AU - Conner, S. J.

AU - Salpekar, A.

AU - Olufowobi, O.

AU - Ashton, P.

AU - Pavlovic, B.

AU - Lenton, W.

AU - Afnan, M.

AU - Brewis, I. A.

AU - Monk, M.

AU - Hughes, D. C.

AU - Barratt, C. L. R.

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model

AB - Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Computational Biology/methods

KW - Egg Proteins/genetics

KW - Female

KW - Gene Expression

KW - Humans

KW - Membrane Glycoproteins/genetics

KW - Mice/genetics

KW - Molecular Sequence Data

KW - Oocytes/metabolism

KW - Proteomics

KW - Receptors, Cell Surface/genetics

KW - Zona Pellucida/metabolism

KW - Zona Pellucida Glycoproteins

U2 - 10.1093/humrep/deh301

DO - 10.1093/humrep/deh301

M3 - Article

C2 - 15142998

SN - 0268-1161

VL - 19

SP - 1580

EP - 1586

JO - Human Reproduction

JF - Human Reproduction

IS - 7

ER -

Four zona pellucida glycoproteins are expressed in the human

Abstract

Keywords

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