Fully automated analysis of Carbohydrate-Deficient Transferrin (CDT) by using a multicapillary electrophoresis system

Federica Bortolotti, Giorgia De Paoli, Jennifer P. Pascali, Franco Tagliaro

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    Background: The techniques universally believed as most reliable for determination of Carbohydrate-Deficient Transferrin (CDT) are HPLC and capillary electrophoresis (CE). Recently a reagent kit for CDT analysis to be used in a multicapillary electropherograph has been introduced. The present work was aimed at a validation of this new commercial system by application of the analytical chemistry parameters and by comparison with validated CE and HPLC methods.

    Methods: One hundred forty serum samples were analyzed with multicapillary CE (Capillarys (TM), Sebia, France) using kit reagents (CAPILLARYS (TM) CDT assay), with single capillary electropherograph (P/ACE MDQ, Beckman Coulter, USA) using an original method developed by our group and with HPLC, performed on a gradient HPLC (Shimadzu Europe, Germany), using column and reagents provided in kit (ClinRelig, Recipe, Germany).

    Results: The separation efficiency of the multicapillary system was about 15,000 theoretical plates/column. The resolution of the transferrin isoforms ranged from 1.23 to 1.67. The variation coefficient was < 10% in both intra-run and between run tests. The correlation of results of multicapillary CE with those obtained with single CE and HPLC was statistically significant.

    Conclusions: The multicapillary system shows high productivity with good analytical performances; however, a confirmation of positive results with an alternative method is required. (c) 2007 Elsevier B.V All rights reserved.

    Original languageEnglish
    Pages (from-to)4-7
    Number of pages4
    JournalClinica Chimica Acta
    Issue number1-2
    Publication statusPublished - 2007

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