Projects per year
Abstract
The β-propeller protein Sec13 plays roles in at least three distinct processes by virtue of being a component of the COPII endoplasmic reticulum export vesicle coat, the nuclear pore complex (NPC) and the Seh1-associated (SEA)/GATOR nutrient-sensing complex. This suggests that regulatory mechanisms coordinating these cellular activities may operate via Sec13. The NPC, COPII and SEA/GATOR are all ancient features of eukaryotic cells, and in the vast majority of eukaryotes, a single Sec13 gene is present. Here we report that the Euglenozoa, a lineage encompassing the diplonemid, kinetoplastid and euglenid protists, possess two Sec13 paralogues. Furthermore, based on protein interactions and localization studies we show that in diplonemids Sec13 functions are divided between the Sec13a and Sec13b paralogues. Specifically, Sec13a interacts with COPII and the NPC, while Sec13b interacts with Sec16 and components of the SEA/GATOR complex. We infer that euglenozoan Sec13a is responsible for NPC functions and canonical anterograde transport activities while Sec13b acts within nutrient and autophagy-related pathways, indicating a fundamentally distinct organization of coatomer complexes in euglenozoan flagellates.
Original language | English |
---|---|
Article number | 220364 |
Number of pages | 15 |
Journal | Open Biology |
Volume | 13 |
Issue number | 6 |
Early online date | 14 Jun 2023 |
DOIs | |
Publication status | Published - 14 Jun 2023 |
Keywords
- Diplonema
- Euglenozoa
- coatomer
- membrane trafficking
- Sec13
- nuclear pore complex
- SEA/GATOR complex
- paralog expansion
- paralogue expansion
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
- General Neuroscience
- Immunology
Fingerprint
Dive into the research topics of 'Functional differentiation of Sec13 paralogues in the euglenozoan protists'. Together they form a unique fingerprint.Projects
- 1 Finished
-
A Systems Approach for Understanding Cell Surface Dynamics in Trypanosomes (Investigator Award)
Field, M. (Investigator)
1/10/17 → 31/03/24
Project: Research