Fundamentals of HDX-MS

Vanesa Vinciauskaite, Glenn R. Masson (Lead / Corresponding author)

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Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.

Original languageEnglish
Number of pages14
JournalEssays in Biochemistry
Early online date17 Oct 2022
Publication statusE-pub ahead of print - 17 Oct 2022


  • HDX-MS
  • LC-MS
  • molecular interactions


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