Fundamentals of HDX-MS

Vanesa Vinciauskaite, Glenn R. Masson (Lead / Corresponding author)

    Research output: Contribution to journalReview articlepeer-review

    22 Citations (Scopus)
    136 Downloads (Pure)

    Abstract

    Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.

    Original languageEnglish
    Pages (from-to)301-314
    Number of pages14
    JournalEssays in Biochemistry
    Volume67
    Issue number2
    Early online date17 Oct 2022
    DOIs
    Publication statusPublished - 29 Mar 2023

    Keywords

    • HDX-MS
    • LC-MS
    • molecular interactions

    ASJC Scopus subject areas

    • Molecular Biology
    • Biochemistry

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