Fundamentals of HDX-MS

Vanesa Vinciauskaite; Glenn R. Masson

doi:10.1042/EBC20220111

Fundamentals of HDX-MS

Vanesa Vinciauskaite
, Glenn R. Masson (Lead / Corresponding author)

Research output: Contribution to journal › Review article › peer-review

46 Citations (Scopus)

267 Downloads (Pure)

Abstract

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.

Original language	English
Pages (from-to)	301-314
Number of pages	14
Journal	Essays in Biochemistry
Volume	67
Issue number	2
Early online date	17 Oct 2022
DOIs	https://doi.org/10.1042/EBC20220111
Publication status	Published - 29 Mar 2023

Keywords

HDX-MS
LC-MS
molecular interactions

ASJC Scopus subject areas

Molecular Biology
Biochemistry

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10.1042/EBC20220111Licence: CC BY

Final published versionFinal published version, 2.35 MBLicence: CC BY

Cite this

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title = "Fundamentals of HDX-MS",

abstract = "Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.",

keywords = "HDX-MS, LC-MS, molecular interactions",

author = "Vanesa Vinciauskaite and Masson, \{Glenn R.\}",

note = "Funding Information: This work was supported by an MRC iCase Studentship [grant number MR/R01579/1. Copyright: {\textcopyright} 2022 The Author(s).",

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journal = "Essays in Biochemistry",

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AU - Masson, Glenn R.

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N2 - Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.

AB - Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of automation in all stages of the technique and its versatility of application-many proteins that present challenges with techniques such as X-ray crystallography and cryoelectron microscopy are amenable to investigation with HDX-MS. The present review is aimed at scientists who are curious about the technique, and how it may aid their research. It describes the fundamental basis of solvent exchange, the basics of a standard HDX-MS experiment, as well as highlighting emerging novel experimental advances, which point to where the field is heading.

KW - HDX-MS

KW - LC-MS

KW - molecular interactions

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U2 - 10.1042/EBC20220111

DO - 10.1042/EBC20220111

M3 - Review article

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VL - 67

SP - 301

EP - 314

JO - Essays in Biochemistry

JF - Essays in Biochemistry

IS - 2

ER -

Fundamentals of HDX-MS

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ASJC Scopus subject areas

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Biophysical characterization of GCN2 kinase: Linking function and structure for drug design

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Fundamentals of HDX-MS

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Keywords

ASJC Scopus subject areas

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Student theses

Biophysical characterization of GCN2 kinase: Linking function and structure for drug design

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