Further studies on the role of glycogenin in glycogen biosynthesis

Carl Smythe, Peter Watt, Philip Cohen

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    About 90% of the glycogenin in skeleta muscle extracts prepared from fed, 24‐h starved or alloxan‐diabetic rabbits sedimented at 140 000xg with the glycogen/sarcovesicular fraction, from which it was released by glycogenolysis, but not by 1% SDS. Glycogenin in the glycogen/sarcovesicular fraction is therefore bound covalently to glycogen, and not associated (covalently or non‐covalently) with the sarcoplasmic reticulum. The same proportion of glycogen synthase was also recovered in the glycogen/sarcovesicle fraction, was solubilised by glycogenolysis, and copurified with glycogenin to yield a heterodimer composed of a 1:1 complex between these proteins. Glycogen synthase and glycogenin are therefore present in equimolar amounts in skeletal muscle and there is an average of one glycogen synthase catalytic subunit associated with each molecule of glycogen in vivo. Glycogenin and glycogen synthase released into the muscle cytosol by degradation of glycogen did not form a complex initially, and only 50% reassociation took place after storage for several hours or overnight dialysis. This suggests that the muscle cytosol may contain a factor(s) which regulates glycogen biogenesis by modulating the association of glycogenin and glycogen synthase. Only glycogen synthase that was complexed to glycogenin was capable of elongating the primer formed by incubation of glycogenin with Mn2+and micromolar concentration of UDP‐glucose, demonstrating the critical importance of this complex for glycogen biogenesis.

    Original languageEnglish
    Pages (from-to)199-204
    Number of pages6
    JournalEuropean Journal of Biochemistry
    Volume189
    Issue number1
    DOIs
    Publication statusPublished - Apr 1990

    Fingerprint Dive into the research topics of 'Further studies on the role of glycogenin in glycogen biosynthesis'. Together they form a unique fingerprint.

    Cite this