FY is an RNA 3 ' end-processing factor that interacts with FCA to control the Arabidopsis floral transition

Gordon G. Simpson, Paul P. Dijkwel, Victor Quesada, Ian R. Henderson, Caroline Dean

    Research output: Contribution to journalArticlepeer-review

    316 Citations (Scopus)

    Abstract

    The nuclear RNA binding protein, FCA, promotes Arabidopsis reproductive development. FCA contains a WW protein interaction domain that is essential for FCA function. We have identified FY as a protein partner for this domain. FY belongs to a highly conserved group of eukaryotic proteins represented in Saccharomyces cerevisiae by the RNA 3' end-processing factor, Pfs2p. FY regulates RNA 3' end processing in Arabidopsis as evidenced through its role in FCA regulation. FCA expression is autoregulated through the use of different polyadenylation sites within the FCA pre-mRNA, and the FCA/FY interaction is required for efficient selection of the promoter-proximal polyadenylation site. The FCA/FY interaction is also required for the downregulation of the floral repressor FLC. We propose that FCA controls 3' end formation of specific transcripts and that in higher eukaryotes, proteins homologous to FY may have evolved as sites of association for regulators of RNA 3' end processing.
    Original languageEnglish
    Pages (from-to)777-787
    Number of pages11
    JournalCell
    Volume113
    Issue number6
    DOIs
    Publication statusPublished - 2003

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