G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus

K Labib, JFX Diffley, SE Kearsey

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    161 Citations (Scopus)

    Abstract

    Cyclin-dependent kinases (CDKs) activate the firing of replication origins during the S phase of the cell cycle, They also block re-initiation of DNA replication within a single cell cycle, by preventing the assembly of prereplicative complexes at origins. We show here that, in budding yeast, CDKs exclude the essential prereplicative-complex component Mcm4 from the nucleus. Although origin firing can be triggered by the B-type cyclins only, both G1-phase and B-type cyclins cause exit of Mcm4 from the nucleus. These results suggest that G1 cyclins may diminish the cell's capacity to assemble prereplicative complexes before B-type cyclins trigger origin firing during S phase.

    Original languageEnglish
    Pages (from-to)415-422
    Number of pages8
    JournalNature Cell Biology
    Volume1
    Issue number7
    DOIs
    Publication statusPublished - Nov 1999

    Keywords

    • FISSION YEAST
    • G1 CYCLINS
    • PROTEIN
    • BUDDING YEAST
    • S-PHASE
    • CELL-CYCLE
    • SACCHAROMYCES-CEREVISIAE
    • INITIATION
    • ORIGINS
    • COMPLEXES

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