TY - JOUR
T1 - GABAB receptors in Schwann cells influence proliferation and myelin protein expression
AU - Magnaghi, Valerio
AU - Ballabio, Marinella
AU - Cavarretta, Ilaria T R
AU - Froestl, Wolfgang
AU - Lambert, Jeremy J.
AU - Zucchi, Ileana
AU - Melcangi, Roberto C.
PY - 2004/5
Y1 - 2004/5
N2 - The location and the role of γ-aminobutyric acid type B (GABA B) receptors in the central nervous system have recently received considerable attention, whilst relatively little is known regarding the peripheral nervous system. In this regard, here we demonstrate for the first time that GABAB receptor isoforms [i.e. GABAB(1) and GABAB(2)] are specifically localized in the rat Schwann cell population of the sciatic nerve. Using the selective GABAB agonist [i.e. (-)-baclofen] and the antagonists (i.e. CGP 62349, CGP 56999 A, CGP 55845 A), such receptors are shown to be functionally active and negatively coupled to the adenylate cyclase system. Furthermore, exposure of cultured Schwann cells to (-)-baclofen inhibits their proliferation and reduces the synthesis of specific myelin proteins (i.e. glycoprotein Po, peripheral myelin protein 22, myelin-associated glycoprotein, connexin 32), providing evidence for a physiological role of GABAB receptors in the glial cells of the peripheral nervous system.
AB - The location and the role of γ-aminobutyric acid type B (GABA B) receptors in the central nervous system have recently received considerable attention, whilst relatively little is known regarding the peripheral nervous system. In this regard, here we demonstrate for the first time that GABAB receptor isoforms [i.e. GABAB(1) and GABAB(2)] are specifically localized in the rat Schwann cell population of the sciatic nerve. Using the selective GABAB agonist [i.e. (-)-baclofen] and the antagonists (i.e. CGP 62349, CGP 56999 A, CGP 55845 A), such receptors are shown to be functionally active and negatively coupled to the adenylate cyclase system. Furthermore, exposure of cultured Schwann cells to (-)-baclofen inhibits their proliferation and reduces the synthesis of specific myelin proteins (i.e. glycoprotein Po, peripheral myelin protein 22, myelin-associated glycoprotein, connexin 32), providing evidence for a physiological role of GABAB receptors in the glial cells of the peripheral nervous system.
KW - GABAB receptors
KW - Myelin
KW - Peripheral glia
KW - Rat
KW - Sciatic nerve
U2 - 10.1111/j.0953-816X.2004.03368.x
DO - 10.1111/j.0953-816X.2004.03368.x
M3 - Article
C2 - 15147298
AN - SCOPUS:2942624409
SN - 0953-816X
VL - 19
SP - 2641
EP - 2649
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 10
ER -