GABAB receptors in Schwann cells influence proliferation and myelin protein expression

TY - JOUR

T1 - GABAB receptors in Schwann cells influence proliferation and myelin protein expression

AU - Magnaghi, Valerio

AU - Ballabio, Marinella

AU - Cavarretta, Ilaria T R

AU - Froestl, Wolfgang

AU - Lambert, Jeremy J.

AU - Zucchi, Ileana

AU - Melcangi, Roberto C.

PY - 2004/5

Y1 - 2004/5

N2 - The location and the role of γ-aminobutyric acid type B (GABA B) receptors in the central nervous system have recently received considerable attention, whilst relatively little is known regarding the peripheral nervous system. In this regard, here we demonstrate for the first time that GABAB receptor isoforms [i.e. GABAB(1) and GABAB(2)] are specifically localized in the rat Schwann cell population of the sciatic nerve. Using the selective GABAB agonist [i.e. (-)-baclofen] and the antagonists (i.e. CGP 62349, CGP 56999 A, CGP 55845 A), such receptors are shown to be functionally active and negatively coupled to the adenylate cyclase system. Furthermore, exposure of cultured Schwann cells to (-)-baclofen inhibits their proliferation and reduces the synthesis of specific myelin proteins (i.e. glycoprotein Po, peripheral myelin protein 22, myelin-associated glycoprotein, connexin 32), providing evidence for a physiological role of GABAB receptors in the glial cells of the peripheral nervous system.

AB - The location and the role of γ-aminobutyric acid type B (GABA B) receptors in the central nervous system have recently received considerable attention, whilst relatively little is known regarding the peripheral nervous system. In this regard, here we demonstrate for the first time that GABAB receptor isoforms [i.e. GABAB(1) and GABAB(2)] are specifically localized in the rat Schwann cell population of the sciatic nerve. Using the selective GABAB agonist [i.e. (-)-baclofen] and the antagonists (i.e. CGP 62349, CGP 56999 A, CGP 55845 A), such receptors are shown to be functionally active and negatively coupled to the adenylate cyclase system. Furthermore, exposure of cultured Schwann cells to (-)-baclofen inhibits their proliferation and reduces the synthesis of specific myelin proteins (i.e. glycoprotein Po, peripheral myelin protein 22, myelin-associated glycoprotein, connexin 32), providing evidence for a physiological role of GABAB receptors in the glial cells of the peripheral nervous system.

KW - GABAB receptors

KW - Myelin

KW - Peripheral glia

KW - Rat

KW - Sciatic nerve

U2 - 10.1111/j.0953-816X.2004.03368.x

DO - 10.1111/j.0953-816X.2004.03368.x

M3 - Article

C2 - 15147298

AN - SCOPUS:2942624409

SN - 0953-816X

VL - 19

SP - 2641

EP - 2649

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

IS - 10

ER -