Gastrin sensitivity of primary human colorectal cancer

the effect of gastrin receptor antagonism

S. A. Watson, T. Clifford, R. E. Sykes, E. Robinson, R. J. C. Steele

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P
    Original languageEnglish
    Pages (from-to)2086-2092
    Number of pages7
    JournalEuropean Journal of Cancer
    Volume31
    Issue number12
    DOIs
    Publication statusPublished - 1995

    Fingerprint

    Cholecystokinin B Receptor
    Gastrins
    Colorectal Neoplasms
    Growth
    Avidin
    Biotin
    Collagen Type I
    Thymidine
    Neoplasms
    Adenocarcinoma
    Fibroblasts
    Monoclonal Antibodies
    CR 2093

    Keywords

    • Amino Acids
    • Cell Division
    • Colorectal Neoplasms
    • Gastrins
    • Hormone Antagonists
    • Hormones
    • Humans
    • Immunoenzyme Techniques
    • Receptors, Cholecystokinin
    • Thymidine
    • Tumor Cells, Cultured

    Cite this

    Watson, S. A. ; Clifford, T. ; Sykes, R. E. ; Robinson, E. ; Steele, R. J. C. / Gastrin sensitivity of primary human colorectal cancer : the effect of gastrin receptor antagonism. In: European Journal of Cancer. 1995 ; Vol. 31, No. 12. pp. 2086-2092.
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    abstract = "The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47{\%}) tumours. CR2093 significantly reversed this stimulated growth (P",
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    author = "Watson, {S. A.} and T. Clifford and Sykes, {R. E.} and E. Robinson and Steele, {R. J. C.}",
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    Gastrin sensitivity of primary human colorectal cancer : the effect of gastrin receptor antagonism. / Watson, S. A.; Clifford, T.; Sykes, R. E.; Robinson, E.; Steele, R. J. C.

    In: European Journal of Cancer, Vol. 31, No. 12, 1995, p. 2086-2092.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Gastrin sensitivity of primary human colorectal cancer

    T2 - the effect of gastrin receptor antagonism

    AU - Watson, S. A.

    AU - Clifford, T.

    AU - Sykes, R. E.

    AU - Robinson, E.

    AU - Steele, R. J. C.

    PY - 1995

    Y1 - 1995

    N2 - The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P

    AB - The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P

    KW - Amino Acids

    KW - Cell Division

    KW - Colorectal Neoplasms

    KW - Gastrins

    KW - Hormone Antagonists

    KW - Hormones

    KW - Humans

    KW - Immunoenzyme Techniques

    KW - Receptors, Cholecystokinin

    KW - Thymidine

    KW - Tumor Cells, Cultured

    U2 - 10.1016/0959-8049(95)00451-3

    DO - 10.1016/0959-8049(95)00451-3

    M3 - Article

    VL - 31

    SP - 2086

    EP - 2092

    JO - European Journal of Cancer

    JF - European Journal of Cancer

    SN - 0959-8049

    IS - 12

    ER -