Gastrin sensitivity of primary human colorectal cancer: the effect of gastrin receptor antagonism

S. A. Watson; T. Clifford; R. E. Sykes; E. Robinson; R. J. C. Steele

doi:10.1016/0959-8049(95)00451-3

Gastrin sensitivity of primary human colorectal cancer: the effect of gastrin receptor antagonism

S. A. Watson, T. Clifford, R. E. Sykes, E. Robinson, R. J. C. Steele

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P

Original language	English
Pages (from-to)	2086-2092
Number of pages	7
Journal	European Journal of Cancer
Volume	31
Issue number	12
DOIs	https://doi.org/10.1016/0959-8049(95)00451-3
Publication status	Published - 1995

Keywords

Amino Acids
Cell Division
Colorectal Neoplasms
Gastrins
Hormone Antagonists
Hormones
Humans
Immunoenzyme Techniques
Receptors, Cholecystokinin
Thymidine
Tumor Cells, Cultured

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0959-8049(95)00451-3

Cite this

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title = "Gastrin sensitivity of primary human colorectal cancer: the effect of gastrin receptor antagonism",

abstract = "The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P ",

keywords = "Amino Acids, Cell Division, Colorectal Neoplasms, Gastrins, Hormone Antagonists, Hormones, Humans, Immunoenzyme Techniques, Receptors, Cholecystokinin, Thymidine, Tumor Cells, Cultured",

author = "Watson, {S. A.} and T. Clifford and Sykes, {R. E.} and E. Robinson and Steele, {R. J. C.}",

year = "1995",

doi = "10.1016/0959-8049(95)00451-3",

language = "English",

volume = "31",

pages = "2086--2092",

journal = "European Journal of Cancer",

issn = "0959-8049",

publisher = "Elsevier",

number = "12",

}

TY - JOUR

T1 - Gastrin sensitivity of primary human colorectal cancer

T2 - the effect of gastrin receptor antagonism

AU - Watson, S. A.

AU - Clifford, T.

AU - Sykes, R. E.

AU - Robinson, E.

AU - Steele, R. J. C.

PY - 1995

Y1 - 1995

N2 - The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P

AB - The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P

KW - Amino Acids

KW - Cell Division

KW - Colorectal Neoplasms

KW - Gastrins

KW - Hormone Antagonists

KW - Hormones

KW - Humans

KW - Immunoenzyme Techniques

KW - Receptors, Cholecystokinin

KW - Thymidine

KW - Tumor Cells, Cultured

U2 - 10.1016/0959-8049(95)00451-3

DO - 10.1016/0959-8049(95)00451-3

M3 - Article

C2 - 8562170

SN - 0959-8049

VL - 31

SP - 2086

EP - 2092

JO - European Journal of Cancer

JF - European Journal of Cancer

IS - 12

ER -

Gastrin sensitivity of primary human colorectal cancer: the effect of gastrin receptor antagonism

Abstract

Keywords

UN SDGs

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