Gastrin sensitivity of primary human colorectal cancer: the effect of gastrin receptor antagonism

S. A. Watson, T. Clifford, R. E. Sykes, E. Robinson, R. J. C. Steele

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    Abstract

    The purpose of this study was to determine the effect of the gastrin receptor antagonist, CR2093, on basal and gastrin-stimulated growth of primary human colorectal adenocarcinomas and to relate this to gastrin receptor expression. Tumour cells, derived from surgical specimens by enzymatic disaggregation, were grown on matrices of type I collagen and irradiated fibroblasts. Gastrin receptor expression was measured by using a mouse monoclonal antibody directed against the gastrin receptor and an avidin-biotin immunocytochemical method. Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. CR2093 significantly reversed this stimulated growth (P
    Original languageEnglish
    Pages (from-to)2086-2092
    Number of pages7
    JournalEuropean Journal of Cancer
    Volume31
    Issue number12
    DOIs
    Publication statusPublished - 1995

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    Keywords

    • Amino Acids
    • Cell Division
    • Colorectal Neoplasms
    • Gastrins
    • Hormone Antagonists
    • Hormones
    • Humans
    • Immunoenzyme Techniques
    • Receptors, Cholecystokinin
    • Thymidine
    • Tumor Cells, Cultured

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