Gene-based sequence diversity analysis of field pea (Pisum)

Runchun Jing, Richard Johnson, Andrea Seres, Gyorgy Kiss, Mike J. Ambrose, Maggie R. Knox, T. H. Noel Ellis, Andrew J. Flavell

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    55 Citations (Scopus)

    Abstract

    Sequence diversity of 39 dispersed gene loci was analyzed in 48 diverse individuals representative of the germs Pisum. The different genes show large variation in diversity parameters, suggesting widely differing levels of selection and a high overall diversity level for the species. The data set yields a genetic diversity tree whose deep branches, involving wild samples, are preserved in a tree derived front a polymorphic retrotransposon insertions in an identical sample set. ThuS, gene regions and intergenic "junk DNA" share a consistent, picture for the genomic diversity of Pisum, despite low, linkage disequilibrium in wild and landrace germplasm, which might be expected to allow independent evolution of these very different DNA classes. Additional lines of evidence indicate that recombination has shuffled gene haplotypes efficiently within Pisum, despite its high level of inbreeding and widespread geographic distribution. Trees derived from individual gene loci show marked differences front each other, and genetic distance values between sample pairs show high standard deviations. Seqnence mosaic analysis of aligned seqnences identifies mile loci showing evidence for intragenic recombination. Lastly, phylogenetic network analysis confirms the non-treelike Structure of Pisum diversity and indicates the major germlasm classes involved. Overall, these data emphasize the artificiality of simple tree Structures for representing genomic sequence variation within Pisum and emphasize the need for fine structure haplotype analysis to accurately define the genetic Structure of the species.

    Original languageEnglish
    Pages (from-to)2263-2275
    Number of pages13
    JournalGenetics
    Volume177
    Issue number4
    DOIs
    Publication statusPublished - Dec 2007

    Keywords

    • LINKAGE DISEQUILIBRIUM
    • ARABIDOPSIS-THALIANA
    • SATIVUM L.
    • PHYLOGENETIC NETWORKS
    • MUTATION DETECTION
    • HORDEUM-VULGARE
    • RECOMBINATION
    • DNA
    • POLYMORPHISM
    • MAIZE

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