Gene deletions leading to a reduction in the number of cyclopentane rings in Sulfolobus acidocaldarius tetraether lipids

Ziqiang Guan, Antonia Delago, Phillip Nußbaum, Benjamin H Meyer, Sonja-Verena Albers, Jerry Eichler (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
127 Downloads (Pure)

Abstract

The cell membrane of (hyper)thermophilic archaea, including the thermoacidophile Sulfolobus acidocaldarius, incorporates dibiphytanylglycerol tetraether lipids. The hydrophobic cores of such tetraether lipids can include up to eight cyclopentane rings. Presently, nothing is known of the biosynthesis of these rings. In this study, a series of S. acidocaldarius mutants deleted of genes currently annotated as encoding proteins involved in sugar/polysaccharide processing were generated and their glycolipids were considered. Whereas the glycerol-dialkyl-glycerol tetraether core of a S. acidocaldarius tetraether glycolipid considered here mostly includes four cyclopentane rings, in cells where the Saci_0421 or Saci_1201 genes had been deleted, species containing zero, two or four cyclopentane rings were observed. At the same time, in cells lacking Saci_0201, Saci_0275, Saci_1101, Saci_1249 or Saci_1706, lipids containing mostly four cyclopentane rings were detected. Although Saci_0421 and Saci_1201 are not found in proximity to other genes putatively involved in lipid biosynthesis, homologs of these sequences exist in other Archaea containing cyclopentane-containing tetraether lipids. Thus, Saci_0421 and Saci_1201 represent the first proteins described that somehow contribute to the appearance of cyclopentane rings in the core moiety of the S. acidocaldarius glycolipid considered here.

Original languageEnglish
Article numberfnx250
Pages (from-to)1-8
Number of pages8
JournalFEMS Microbiology Letters
Volume365
Issue number1
Early online date29 Nov 2017
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Journal Article
  • Archaea
  • cyclopentane rings
  • glycerol-dialkyl-glycerol tetraether (GDGT)
  • glycolipids
  • mass spectrometry
  • Sulfolobus acidocaldarius
  • tetraether lipids

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