Gene expression profiling of archival tongue squamous cell carcinomas provides sub-classification based on DNA repair genes

Matilda Rentoft, Göran Laurell, Philip John Coates, Björn Sjöström, Karin Nylander

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    A subgroup of patients with squamous cell carcinoma of the head and neck (SCCHN) comprise young persons under the age of 40, who have not been heavily exposed to the classical risk factors, smoking and alcohol. The number of SCCHN in young adults, particularly tongue tumours, is increasing in several parts of the world. Here we employed a novel gene expression array methodology specifically developed for analysis of degraded RNA and investigated the expression of 502 cancer-related genes in archival paraffin-embedded SCCHN of the tongue from young (<or =40) and elderly patients (> or =50). Genes detected as de-regulated in tumours compared to non-malignant controls were in concordance with results from earlier studies of fresh frozen material. No genes were detected as significantly differentially expressed between young and old patients suggesting that the overall pathobiology of SCCHN is similar in young and old. Unsupervised clustering divided tumours into three groups, irrespective of age, where several differentially expressed DNA repair genes were a prominent separation factor. High levels of DNA repair genes associated with impaired therapeutic response to radiation, suggesting that DNA repair genes play a role in clinical outcome after radiotherapy.
    Original languageEnglish
    Pages (from-to)1321-30
    Number of pages10
    JournalInternational Journal of Oncology
    Volume35
    Issue number6
    DOIs
    Publication statusPublished - Dec 2009

    Fingerprint

    Gene Expression Profiling
    Tongue
    DNA Repair
    Squamous Cell Carcinoma
    Genes
    Neoplasms
    Neoplasm Genes
    Paraffin
    Cluster Analysis
    Young Adult
    Radiotherapy
    Age Groups
    Smoking
    Alcohols
    RNA
    Radiation
    Gene Expression
    Carcinoma, squamous cell of head and neck

    Cite this

    Rentoft, Matilda ; Laurell, Göran ; Coates, Philip John ; Sjöström, Björn ; Nylander, Karin. / Gene expression profiling of archival tongue squamous cell carcinomas provides sub-classification based on DNA repair genes. In: International Journal of Oncology. 2009 ; Vol. 35, No. 6. pp. 1321-30.
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    abstract = "A subgroup of patients with squamous cell carcinoma of the head and neck (SCCHN) comprise young persons under the age of 40, who have not been heavily exposed to the classical risk factors, smoking and alcohol. The number of SCCHN in young adults, particularly tongue tumours, is increasing in several parts of the world. Here we employed a novel gene expression array methodology specifically developed for analysis of degraded RNA and investigated the expression of 502 cancer-related genes in archival paraffin-embedded SCCHN of the tongue from young ( or =50). Genes detected as de-regulated in tumours compared to non-malignant controls were in concordance with results from earlier studies of fresh frozen material. No genes were detected as significantly differentially expressed between young and old patients suggesting that the overall pathobiology of SCCHN is similar in young and old. Unsupervised clustering divided tumours into three groups, irrespective of age, where several differentially expressed DNA repair genes were a prominent separation factor. High levels of DNA repair genes associated with impaired therapeutic response to radiation, suggesting that DNA repair genes play a role in clinical outcome after radiotherapy.",
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    Gene expression profiling of archival tongue squamous cell carcinomas provides sub-classification based on DNA repair genes. / Rentoft, Matilda; Laurell, Göran; Coates, Philip John; Sjöström, Björn; Nylander, Karin.

    In: International Journal of Oncology, Vol. 35, No. 6, 12.2009, p. 1321-30.

    Research output: Contribution to journalArticle

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    AU - Laurell, Göran

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    AU - Nylander, Karin

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    AB - A subgroup of patients with squamous cell carcinoma of the head and neck (SCCHN) comprise young persons under the age of 40, who have not been heavily exposed to the classical risk factors, smoking and alcohol. The number of SCCHN in young adults, particularly tongue tumours, is increasing in several parts of the world. Here we employed a novel gene expression array methodology specifically developed for analysis of degraded RNA and investigated the expression of 502 cancer-related genes in archival paraffin-embedded SCCHN of the tongue from young ( or =50). Genes detected as de-regulated in tumours compared to non-malignant controls were in concordance with results from earlier studies of fresh frozen material. No genes were detected as significantly differentially expressed between young and old patients suggesting that the overall pathobiology of SCCHN is similar in young and old. Unsupervised clustering divided tumours into three groups, irrespective of age, where several differentially expressed DNA repair genes were a prominent separation factor. High levels of DNA repair genes associated with impaired therapeutic response to radiation, suggesting that DNA repair genes play a role in clinical outcome after radiotherapy.

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