TY - CHAP
T1 - Gene-targeting vectors
AU - Simon, J.
AU - Arthur, C.
AU - McGuire, Victoria A.
PY - 2009/5
Y1 - 2009/5
N2 - Gene targeting in mice has been used extensively to elucidate gene function in vivo. However, for gene targeting to be successful, the targeting vector must be carefully designed. This chapter addresses the rationale behind designing targeting vectors, detailing the essential components, and highlighting specific considerations for different types of vectors, from gene deletions to point mutations and insertions. Examples of vector designs, cloning strategies, and approaches for successful screening of recombinants are described. The use of Cre/LoxP and Flp/frt systems for conditional targeting is described, together with strategies for generating conditional deletions. Methods for generating conditional point mutations are also described and their potential drawbacks discussed.
AB - Gene targeting in mice has been used extensively to elucidate gene function in vivo. However, for gene targeting to be successful, the targeting vector must be carefully designed. This chapter addresses the rationale behind designing targeting vectors, detailing the essential components, and highlighting specific considerations for different types of vectors, from gene deletions to point mutations and insertions. Examples of vector designs, cloning strategies, and approaches for successful screening of recombinants are described. The use of Cre/LoxP and Flp/frt systems for conditional targeting is described, together with strategies for generating conditional deletions. Methods for generating conditional point mutations are also described and their potential drawbacks discussed.
KW - Cre
KW - Frt
KW - Gene-targeting vector
KW - Knockin
KW - Knockout
UR - http://www.scopus.com/inward/record.url?scp=70349254330&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-019-9_9
DO - 10.1007/978-1-60327-019-9_9
M3 - Chapter
C2 - 19504069
AN - SCOPUS:70349254330
SN - 9781603270182
T3 - Methods in Molecular Biology
SP - 127
EP - 144
BT - Transgenesis techniques
A2 - Cartwright, Elizabeth J.
PB - Springer
ER -