Genetic Analysis of Cytochrome P450 System

Nigel K. Spurr; Alan C. Gough; C. A. Dale Smith; C. Roland Wolf

doi:10.1016/0076-6879(91)06086-I

Genetic Analysis of Cytochrome P450 System

Nigel K. Spurr, Alan C. Gough, C. A. Dale Smith, C. Roland Wolf

Research output: Contribution to journal › Article › peer-review

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Abstract

This chapter discusses the genetic analysis of cytochrome P450 system. Molecular genetic analysis of cytochrome P450 enzymes represents an essential part of studies of the properties of this enzyme system. Such analysis allows the chromosomal assignment of P450 genes and the complexity and organization of specific gene clusters to be determined. This approach can also be used to determine the association of a specific P450 gene with a trait or phenotype, for example, cancer susceptibility, and also for the association of specific gene loci with the metabolism of specific drugs or hormones. Genetic analysis in mammals has been predominantly carried out in humans or the mouse. In the latter case, this is because of the availability of a wide range of inbred lines and the wealth of information already available on mouse genetics. Once a new gene or complementary DNA (cDNA) has been isolated one of the first steps in genetic analysis is to determine the chromosome assignment.

Original language	English
Pages (from-to)	149-166
Number of pages	18
Journal	Methods in Enzymology
Volume	206
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(91)06086-I
Publication status	Published - 1991

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/0076-6879(91)06086-I

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title = "Genetic Analysis of Cytochrome P450 System",

abstract = "This chapter discusses the genetic analysis of cytochrome P450 system. Molecular genetic analysis of cytochrome P450 enzymes represents an essential part of studies of the properties of this enzyme system. Such analysis allows the chromosomal assignment of P450 genes and the complexity and organization of specific gene clusters to be determined. This approach can also be used to determine the association of a specific P450 gene with a trait or phenotype, for example, cancer susceptibility, and also for the association of specific gene loci with the metabolism of specific drugs or hormones. Genetic analysis in mammals has been predominantly carried out in humans or the mouse. In the latter case, this is because of the availability of a wide range of inbred lines and the wealth of information already available on mouse genetics. Once a new gene or complementary DNA (cDNA) has been isolated one of the first steps in genetic analysis is to determine the chromosome assignment.",

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AU - Spurr, Nigel K.

AU - Gough, Alan C.

AU - Dale Smith, C. A.

AU - Roland Wolf, C.

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AB - This chapter discusses the genetic analysis of cytochrome P450 system. Molecular genetic analysis of cytochrome P450 enzymes represents an essential part of studies of the properties of this enzyme system. Such analysis allows the chromosomal assignment of P450 genes and the complexity and organization of specific gene clusters to be determined. This approach can also be used to determine the association of a specific P450 gene with a trait or phenotype, for example, cancer susceptibility, and also for the association of specific gene loci with the metabolism of specific drugs or hormones. Genetic analysis in mammals has been predominantly carried out in humans or the mouse. In the latter case, this is because of the availability of a wide range of inbred lines and the wealth of information already available on mouse genetics. Once a new gene or complementary DNA (cDNA) has been isolated one of the first steps in genetic analysis is to determine the chromosome assignment.

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Genetic Analysis of Cytochrome P450 System

Abstract

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