Genetic differentiation of cocoa (Theobrotna cacao L.) populations revealed by RAPD analysis

J. R. Russell, F. Hosein, E. Johnson, R. Waugh, W. Powell

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

In order to preserve and exploit the valuable genetic resources of tropical forest trees, such as cocoa, a systematic assessment of the available genetic variability is necessary. The approach we have used is based on a simple mini‐prep DNA extraction procedure together with a polymerase‐chain‐reaction‐ (PCR)‐based polymorphic assay procedure (RAPD). Twenty‐five cocoa accessions: IMCs and PAs collected from Peru and LCTEENs collected from Ecuador, which are difficult to distinguish using morphological or biochemical descriptors, were uniquely fingerprinted using a minimum of three oligonucleotide primers. Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations. Partitioning of variability into within and between population components revealed that most variation was detected within a population. The potential of RAPD analysis to facilitate the rationalization of field gene banks and provide accurate estimates of diversity to allow optimization of collecting strategies is discussed

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalMolecular Ecology
Volume2
Issue number2
DOIs
Publication statusPublished - Apr 1993

Fingerprint

cocoa
Cacao
genetic differentiation
assay
Polymerase Chain Reaction
genetic variation
assays
Population
Ecuador
Peru
DNA Primers
rationalization
gene banks
genetic resource
DNA primers
forest trees
genetic resources
tropical forests
tropical forest
preserves

Keywords

  • cocoa
  • DNA fingerprinting
  • genetic diversity
  • population
  • RAPD
  • woody perennial

Cite this

Russell, J. R. ; Hosein, F. ; Johnson, E. ; Waugh, R. ; Powell, W. / Genetic differentiation of cocoa (Theobrotna cacao L.) populations revealed by RAPD analysis. In: Molecular Ecology. 1993 ; Vol. 2, No. 2. pp. 89-97.
@article{f29e94b7fc8240f5a9ed45fb8a638462,
title = "Genetic differentiation of cocoa (Theobrotna cacao L.) populations revealed by RAPD analysis",
abstract = "In order to preserve and exploit the valuable genetic resources of tropical forest trees, such as cocoa, a systematic assessment of the available genetic variability is necessary. The approach we have used is based on a simple mini‐prep DNA extraction procedure together with a polymerase‐chain‐reaction‐ (PCR)‐based polymorphic assay procedure (RAPD). Twenty‐five cocoa accessions: IMCs and PAs collected from Peru and LCTEENs collected from Ecuador, which are difficult to distinguish using morphological or biochemical descriptors, were uniquely fingerprinted using a minimum of three oligonucleotide primers. Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations. Partitioning of variability into within and between population components revealed that most variation was detected within a population. The potential of RAPD analysis to facilitate the rationalization of field gene banks and provide accurate estimates of diversity to allow optimization of collecting strategies is discussed",
keywords = "cocoa, DNA fingerprinting, genetic diversity, population, RAPD, woody perennial",
author = "Russell, {J. R.} and F. Hosein and E. Johnson and R. Waugh and W. Powell",
year = "1993",
month = "4",
doi = "10.1111/j.1365-294X.1993.tb00003.x",
language = "English",
volume = "2",
pages = "89--97",
journal = "Molecular Ecology",
issn = "0962-1083",
publisher = "Wiley",
number = "2",

}

Genetic differentiation of cocoa (Theobrotna cacao L.) populations revealed by RAPD analysis. / Russell, J. R.; Hosein, F.; Johnson, E.; Waugh, R.; Powell, W.

In: Molecular Ecology, Vol. 2, No. 2, 04.1993, p. 89-97.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic differentiation of cocoa (Theobrotna cacao L.) populations revealed by RAPD analysis

AU - Russell, J. R.

AU - Hosein, F.

AU - Johnson, E.

AU - Waugh, R.

AU - Powell, W.

PY - 1993/4

Y1 - 1993/4

N2 - In order to preserve and exploit the valuable genetic resources of tropical forest trees, such as cocoa, a systematic assessment of the available genetic variability is necessary. The approach we have used is based on a simple mini‐prep DNA extraction procedure together with a polymerase‐chain‐reaction‐ (PCR)‐based polymorphic assay procedure (RAPD). Twenty‐five cocoa accessions: IMCs and PAs collected from Peru and LCTEENs collected from Ecuador, which are difficult to distinguish using morphological or biochemical descriptors, were uniquely fingerprinted using a minimum of three oligonucleotide primers. Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations. Partitioning of variability into within and between population components revealed that most variation was detected within a population. The potential of RAPD analysis to facilitate the rationalization of field gene banks and provide accurate estimates of diversity to allow optimization of collecting strategies is discussed

AB - In order to preserve and exploit the valuable genetic resources of tropical forest trees, such as cocoa, a systematic assessment of the available genetic variability is necessary. The approach we have used is based on a simple mini‐prep DNA extraction procedure together with a polymerase‐chain‐reaction‐ (PCR)‐based polymorphic assay procedure (RAPD). Twenty‐five cocoa accessions: IMCs and PAs collected from Peru and LCTEENs collected from Ecuador, which are difficult to distinguish using morphological or biochemical descriptors, were uniquely fingerprinted using a minimum of three oligonucleotide primers. Analysis of the variability detected using RAPDs clearly discriminated between the geographical origin of the three cocoa populations. Partitioning of variability into within and between population components revealed that most variation was detected within a population. The potential of RAPD analysis to facilitate the rationalization of field gene banks and provide accurate estimates of diversity to allow optimization of collecting strategies is discussed

KW - cocoa

KW - DNA fingerprinting

KW - genetic diversity

KW - population

KW - RAPD

KW - woody perennial

UR - http://www.scopus.com/inward/record.url?scp=0027585135&partnerID=8YFLogxK

U2 - 10.1111/j.1365-294X.1993.tb00003.x

DO - 10.1111/j.1365-294X.1993.tb00003.x

M3 - Article

C2 - 8180737

AN - SCOPUS:0027585135

VL - 2

SP - 89

EP - 97

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 2

ER -