Genetic fingerprinting of Theobroma clones using randomly amplified polymorphic DNA markers

J. Wilde, R. Waugh, W. Powell (Lead / Corresponding author)

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Randomly amplified polymorphic DNA (RAPD) markers have been used to characterise cocoa clones representing the three main cultivated subpopulations: Criollo, Forastero and Trinitario. The use of single primers of arbitrary nucleotide sequence resulted in the selective amplification of DNA fragments which were unique to the individual cocoa clones studied. The use of a single primer allowed each of the clones evaluated to be unequivocally characterised. The application of RAPD markers for the evaluation of germplasm and cocoa improvement programmes are discussed.

Original languageEnglish
Pages (from-to)871-877
Number of pages7
JournalTheoretical and Applied Genetics
Volume83
Issue number6-7
DOIs
Publication statusPublished - 1 Apr 1992

Keywords

  • Cocoa
  • DNA
  • Genetic diversity
  • Polymorphism
  • RAPDs

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