Genetic linkage mapping of economically important traits in cultivated tetraploid potato (Solanum tuberosum L.)

Alicia N. Massa, Norma C. Manrique-Carpintero, Joseph J. Coombs, Daniel G. Zarka, Anne E. Boone, William W. Kirk, Christine A. Hackett, Glenn J. Bryan, David S. Douches (Lead / Corresponding author)

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Abstract

The objective of this study was to construct a single nucleotide polymorphism (SNP)-based genetic map at the cultivated tetraploid level to locate quantitative trait loci (QTL) contributing to economically important traits in potato (Solanum tuberosum L.). The 156 F1 progeny and parents of a cross (MSL603) between "Jacqueline Lee" and "MSG227-2" were genotyped using the Infinium 8303 Potato Array. Furthermore, the progeny and parents were evaluated for foliar late blight reaction to isolates of the US-8 genotype of Phytophthora infestans (Mont.) de Bary and vine maturity. Linkage analyses and QTL mapping were performed using a novel approach that incorporates allele dosage information. The resulting genetic maps contained 1972 SNP markers with an average density of 1.36 marker per cM. QTL mapping identified the major source of late blight resistance in "Jacqueline Lee." The best SNP marker mapped ~0.54 Mb from a resistance hotspot on the long arm of chromosome 9. For vine maturity, the major-effect QTL was located on chromosome 5 with allelic effects from both parents. A candidate SNP marker for this trait mapped ~0.25 Mb from the StCDF1 gene, which is a candidate gene for the maturity trait. The identification of markers for P. infestans resistance will enable the introgression of multiple sources of resistance through marker-assisted selection. Moreover, the discovery of a QTL for late blight resistance not linked to the QTL for vine maturity provides the opportunity to use marker-assisted selection for resistance independent of the selection for vine maturity classifications.

Original languageEnglish
Pages (from-to)2357-2364
Number of pages8
JournalG3 : Genes, Genomes, Genetics
Volume5
Issue number11
Early online date14 Sep 2015
DOIs
Publication statusPublished - Nov 2015

Keywords

  • Disease resistance
  • Genetic linkage
  • Phytophthora infestans
  • Polymorphism, Single nucleotide
  • Quantitative trait loci
  • Solanum tuberosum
  • Tetraploidy
  • Journal article
  • Research support, Non-U.S. Gov't
  • Research support, U.S. Gov't, Non-P.H.S.

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    Massa, A. N., Manrique-Carpintero, N. C., Coombs, J. J., Zarka, D. G., Boone, A. E., Kirk, W. W., Hackett, C. A., Bryan, G. J., & Douches, D. S. (2015). Genetic linkage mapping of economically important traits in cultivated tetraploid potato (Solanum tuberosum L.). G3 : Genes, Genomes, Genetics, 5(11), 2357-2364. https://doi.org/10.1534/g3.115.019646