Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

Original languageEnglish
Title of host publicationCryptosporidium
EditorsJan R. Mead, Michael J. Arrowood
Place of PublicationNew York
PublisherHumana Press
Chapter13
Pages219-228
Number of pages10
ISBN (Electronic)9781493997480
ISBN (Print)9781493997473
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
PublisherSpringer Verlag
Volume2052
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CRISPR
  • Cas9
  • Genetics
  • Transfection
  • Transgenics

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  • Cite this

    Sateriale, A., Pawlowic, M., Vinayak, S., Brooks, C., & Striepen, B. (2020). Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In J. R. Mead, & M. J. Arrowood (Eds.), Cryptosporidium (pp. 219-228). (Methods in Molecular Biology; Vol. 2052). Humana Press. https://doi.org/10.1007/978-1-4939-9748-0_13