Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter


Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

Original languageEnglish
Title of host publicationCryptosporidium
EditorsJan R. Mead, Michael J. Arrowood
Place of PublicationNew York
PublisherHumana Press
Number of pages10
ISBN (Electronic)9781493997480
ISBN (Print)9781493997473
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
PublisherSpringer Verlag
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cas9
  • Genetics
  • Transfection
  • Transgenics

Fingerprint Dive into the research topics of 'Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9'. Together they form a unique fingerprint.

  • Cite this

    Sateriale, A., Pawlowic, M., Vinayak, S., Brooks, C., & Striepen, B. (2020). Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In J. R. Mead, & M. J. Arrowood (Eds.), Cryptosporidium (pp. 219-228). (Methods in Molecular Biology; Vol. 2052). Humana Press.