Genetical analysis of in vitro cell and tissue culture response in potato

M. Coleman, R. Waugh, W. Powell

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


The genetics of tissue culture response in potato has been examined by analysing a sample of dihaploids (2n=2x=24) extracted from tetraploid parents (4n=4x=48). The genotypes were screened for rate of nodal multiplication, in vitro tuberisation, regeneration from leaf discs and protoplast plating efficiency. Significant differences were detected between dihaploids for the traits measured and this indicates that tissue culture response in the tetraploid parents must be in the heterozygous condition. Estimates of the broad sense heritabilities were calculated together with the number of genes or effective factors involved in the control of the traits. These estimates indicate that tissue culture response in potato is under relatively simple genetic control and "blocks of genes" may be located on specific chromosomes. The inheritance of RFLP markers in the segregating dihaploid population was also monitored and the potential of using molecular markers linked to gene(s) controlling tissue culture response is discussed.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Issue number3
Publication statusPublished - 1 Dec 1990


  • heritability
  • protoplasts
  • RFLPs
  • Solanum tuberosum

ASJC Scopus subject areas

  • Horticulture


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