Genetically directed production of recombinant, isosteric and non-hydrolyzable ubiquitin conjugates

Mathew Stanley, Satpal Virdee (Lead / Corresponding author)

Research output: Contribution to journalArticle

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27 Downloads (Pure)

Abstract

We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins using a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNACUA pair. This allows the general production of non-hydrolyzable ubiquitin conjugates of recombinant origin via bioorthogonal oxime ligation. This is exemplified by the preparation of non-hydrolyzable versions of diubiquitin, polymeric ubiquitin chains and ubiquitinated SUMO. We demonstrate that the conjugates exhibit unrivalled isostery with the native isopeptide bond through both structural and biophysical characterization. Furthermore, the conjugates function as nanomolar inhibitors of deubiquitinating enzymes and are recognized by linkage–specific antibodies. This technology should provide a versatile platform for the development of powerful research tools for studying deubiquitinating enzymes and for defining the cellular roles of diverse polyubiquitin linkages.
Original languageEnglish
Pages (from-to)1472-1480
Number of pages9
JournalChemBioChem
Volume17
Issue number15
Early online date20 May 2016
DOIs
Publication statusPublished - 27 Jun 2016

Fingerprint

Ubiquitin
Methanosarcina barkeri
Polyubiquitin
Amino Acyl-tRNA Synthetases
Oximes
Enzymes
Recombinant Proteins
Ligation
Technology
Antibodies
Research
Deubiquitinating Enzymes

Keywords

  • ubiquitination
  • oxime
  • genetic code expansion
  • isopeptide
  • isotere
  • deubiquitinating enzyme

Cite this

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AU - Virdee, Satpal

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N2 - We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins using a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNACUA pair. This allows the general production of non-hydrolyzable ubiquitin conjugates of recombinant origin via bioorthogonal oxime ligation. This is exemplified by the preparation of non-hydrolyzable versions of diubiquitin, polymeric ubiquitin chains and ubiquitinated SUMO. We demonstrate that the conjugates exhibit unrivalled isostery with the native isopeptide bond through both structural and biophysical characterization. Furthermore, the conjugates function as nanomolar inhibitors of deubiquitinating enzymes and are recognized by linkage–specific antibodies. This technology should provide a versatile platform for the development of powerful research tools for studying deubiquitinating enzymes and for defining the cellular roles of diverse polyubiquitin linkages.

AB - We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins using a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNACUA pair. This allows the general production of non-hydrolyzable ubiquitin conjugates of recombinant origin via bioorthogonal oxime ligation. This is exemplified by the preparation of non-hydrolyzable versions of diubiquitin, polymeric ubiquitin chains and ubiquitinated SUMO. We demonstrate that the conjugates exhibit unrivalled isostery with the native isopeptide bond through both structural and biophysical characterization. Furthermore, the conjugates function as nanomolar inhibitors of deubiquitinating enzymes and are recognized by linkage–specific antibodies. This technology should provide a versatile platform for the development of powerful research tools for studying deubiquitinating enzymes and for defining the cellular roles of diverse polyubiquitin linkages.

KW - ubiquitination

KW - oxime

KW - genetic code expansion

KW - isopeptide

KW - isotere

KW - deubiquitinating enzyme

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DO - 10.1002/cbic.201600138

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