Genetically directing epsilon-N, N-dimethyl-L-lysine in recombinant histones

Duy P. Nguyen, Maria M. Garcia Alai, Satpal Virdee, Jason W. Chin

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    A molecular understanding of the biological phenomena orchestrated by lysine NE-methylation is impeded by the challenge of producing site-specifically and quantitatively methylated histones Here, we report a general method that combines genetic code expansion and chemoselective reactions, for the quantitative, site-specific installation of dimethyllysine in recombinant histones We demonstrate the utility of our method by preparing H3K9me2 and show that this modified histone is specifically recognized by heterochromatin protein 1 beta Extensions of the strategy reported here will allow a range of chemoselective reactions (which have been used for residue-selective, but not site-selective protein modification) to be leveraged for site-specific protein modification

    Original languageEnglish
    Pages (from-to)1072-1076
    Number of pages5
    JournalChemistry & Biology
    Volume17
    Issue number10
    DOIs
    Publication statusPublished - 29 Oct 2010

    Cite this

    Nguyen, Duy P. ; Alai, Maria M. Garcia ; Virdee, Satpal ; Chin, Jason W. / Genetically directing epsilon-N, N-dimethyl-L-lysine in recombinant histones. In: Chemistry & Biology. 2010 ; Vol. 17, No. 10. pp. 1072-1076.
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    Genetically directing epsilon-N, N-dimethyl-L-lysine in recombinant histones. / Nguyen, Duy P.; Alai, Maria M. Garcia; Virdee, Satpal; Chin, Jason W.

    In: Chemistry & Biology, Vol. 17, No. 10, 29.10.2010, p. 1072-1076.

    Research output: Contribution to journalArticle

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    AU - Alai, Maria M. Garcia

    AU - Virdee, Satpal

    AU - Chin, Jason W.

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