TY - JOUR
T1 - Genome of Leptomonas pyrrhocoris
T2 - a high-quality reference for monoxenous trypanosomatids and new insights into evolution of Leishmania
AU - Flegontov, Pavel
AU - Butenko, Anzhelika
AU - Firsov, Sergei
AU - Kraeva, Natalya
AU - Eliáš, Marek
AU - Field, Mark C.
AU - Filatov, Dmitry
AU - Flegontova, Olga
AU - Gerasimov, Evgeny S.
AU - Hlaváčová, Jana
AU - Ishemgulova, Aygul
AU - Jackson, Andrew P.
AU - Kelly, Steve
AU - Kostygov, Alexei Y.
AU - Logacheva, Maria D.
AU - Maslov, Dmitri A.
AU - Opperdoes, Fred R.
AU - O'Reilly, Amanda
AU - Sádlová, Jovana
AU - Ševčíková, Tereza
AU - Venkatesh, Divya
AU - Vlček, Čestmír
AU - Volf, Petr
AU - Votýpka, Jan
AU - Záhonová, Kristína
AU - Yurchenko, Vyacheslav
AU - Lukeš, Julius
N1 - The financial support through the project LO1208 of the National Feasibility Programme I of the Czech Republic is gratefully appreciated. This work was supported by the following grants: Czech Science Foundation (14-23986S to J.L., 13-24983S and 15-16406S to M.E., and 16-18699S to J.L. and V.Y.), the Moravskoslezský Kraj research initiatives DT1/RRC/2014 (to V.Y., A.K., P.F.) and 00955/RRC/2015 (to V.Y.), the Russian Science Foundation (project 14-50-00029 to M.D.L.), and the Russian Foundation for Basic Research (project 14-04-31936 to P.F. and E.G.).
PY - 2016/3
Y1 - 2016/3
N2 - Many high-quality genomes are available for dixenous (two hosts) trypanosomatid species of the genera Trypanosoma, Leishmania, and Phytomonas, but only fragmentary information is available for monoxenous (single-host) trypanosomatids. In trypanosomatids, monoxeny is ancestral to dixeny, thus it is anticipated that the genome sequences of the key monoxenous parasites will be instrumental for both understanding the origin of parasitism and the evolution of dixeny. Here, we present a high-quality genome for Leptomonas pyrrhocoris, which is closely related to the dixenous genus Leishmania. The L. pyrrhocoris genome (30.4 Mbp in 60 scaffolds) encodes 10,148 genes. Using the L. pyrrhocoris genome, we pinpointed genes gained in Leishmania. Among those genes, 20 genes with unknown function had expression patterns in the Leishmania mexicana life cycle suggesting their involvement in virulence. By combining differential expression data for L. mexicana, L. major and Leptomonas seymouri, we have identified several additional proteins potentially involved in virulence, including SpoU methylase and U3 small nucleolar ribonucleoprotein IMP3. The population genetics of L. pyrrhocoris was also addressed by sequencing thirteen strains of different geographic origin, allowing the identification of 1,318 genes under positive selection. This set of genes was significantly enriched in components of the cytoskeleton and the flagellum.
AB - Many high-quality genomes are available for dixenous (two hosts) trypanosomatid species of the genera Trypanosoma, Leishmania, and Phytomonas, but only fragmentary information is available for monoxenous (single-host) trypanosomatids. In trypanosomatids, monoxeny is ancestral to dixeny, thus it is anticipated that the genome sequences of the key monoxenous parasites will be instrumental for both understanding the origin of parasitism and the evolution of dixeny. Here, we present a high-quality genome for Leptomonas pyrrhocoris, which is closely related to the dixenous genus Leishmania. The L. pyrrhocoris genome (30.4 Mbp in 60 scaffolds) encodes 10,148 genes. Using the L. pyrrhocoris genome, we pinpointed genes gained in Leishmania. Among those genes, 20 genes with unknown function had expression patterns in the Leishmania mexicana life cycle suggesting their involvement in virulence. By combining differential expression data for L. mexicana, L. major and Leptomonas seymouri, we have identified several additional proteins potentially involved in virulence, including SpoU methylase and U3 small nucleolar ribonucleoprotein IMP3. The population genetics of L. pyrrhocoris was also addressed by sequencing thirteen strains of different geographic origin, allowing the identification of 1,318 genes under positive selection. This set of genes was significantly enriched in components of the cytoskeleton and the flagellum.
UR - http://www.scopus.com/inward/record.url?scp=84962425293&partnerID=8YFLogxK
U2 - 10.1038/srep23704
DO - 10.1038/srep23704
M3 - Article
C2 - 27021793
AN - SCOPUS:84962425293
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 23704
ER -