Genome-wide association study for resistance to rhynchosporium in a diverse collection of spring barley germplasm

Jean Noël Thauvin (Lead / Corresponding author), Joanne Russell, Dominique Vequaud, Mark Looseley, Micha Bayer, Pierre Marie Le Roux, Pierre Pin, Robbie Waugh, Anna Avrova (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
47 Downloads (Pure)

Abstract

Rhynchosporium is one of the main biotic stresses on barley production worldwide. A set of 312 spring barley accessions was tested in four different locations over 3 years, to identify novel genetic resistances to rhynchosporium and to explore the allelic diversity for resistance genes present in this global germplasm collection. High-density genotypes from exome capture and RNA-seq were used to conduct high-resolution association mapping. Seven quantitative trait loci (QTL) were detected, including one in the Rrs2 region, amongst five containing known resistances. Relatively short physical intervals harbouring these resistances were proposed, providing a platform for the identification of underlying genes and tightly linked genetic markers for use in marker assisted selection. Genes encoding kinases were present in four of the QTL, in addition to Rrs1 and Rrs18, two loci known to contribute to rhynchosporium resistance. The frequencies and distributions of these novel and known QTL were superimposed on the regional origin of the landrace genotypes comprising the genome-wide association studies (GWAS) panel, highlighting the value of genetic resources as a source of diverse genetically controlled resistance to rhynchosporium. The detected QTL along with their linked genetic markers, could be exploited either directly for breeding pur-poses or for candidate gene identification in future studies.

Original languageEnglish
Article number782
Number of pages14
JournalAgronomy
Volume12
Issue number4
DOIs
Publication statusPublished - 24 Mar 2022

Keywords

  • barley
  • genetic markers
  • GWAS
  • landraces
  • QTL
  • resistance
  • rhynchosporium
  • SNP

ASJC Scopus subject areas

  • Agronomy and Crop Science

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