@inbook{b1192981e0b34e139a0520adf6b9b838,
title = "Genome-Wide Proteomics and Phosphoproteomics Analysis of Leishmania spp. During Differentiation",
abstract = "Determining variations in protein abundance and/or posttranslational modification as a function of time or upon induction by a signal in a particular cell type is central to quantitative proteomics. Isobaric labeling methodologies now allow for parallel quantification of proteins at various conditions concurrently or multiplexing in relatively quantitative proteomics workflows. Hence, mapping the protein expression profiles of various developmental stages of Leishmania parasites is possible with high-resolution mass spectrometry. To analyze global changes in protein expression and cellular signaling pathways during Leishmania differentiation and development is possible with a quantitative proteomics approach. The tandem mass tags (TMT) approach provides a chemical labeling method based on the principle of amine reactive tags; the maximum number of conditions that can be multiplexed is 10-plex. We describe herein a detailed method for sample preparation, TMT-labeling, mass spectrometry and data analysis of different developmental stages of Leishmania donovani parasites. This quantitative proteomic approach is useful to study dynamic changes in protein expression levels during L. donovani differentiation, and also allows in-depth analysis of signaling pathways via phosphoproteomics.",
keywords = "Amastigote, Leishmania development, Parasite differentiation, Promastigote, Proteogenomics, Signaling, Tandem mass tag (TMT)",
author = "Harsh Pawar and Gajanan Sathe and Patole, {Milind S.}",
note = "Funding Information: statement: Dr. Harsh Pawar is funded by an Israel Planning and Budgeting Commission (P.B.C.) fellowship. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0294-2_12",
language = "English",
isbn = "9781071602935",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "161--176",
editor = "Michels, {Paul A. M.} and Ginger, {Michael L.} and Dan Zilberstein",
booktitle = "Trypanosomatids",
address = "United States",
}