Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants

Florian Jupe, Xinwei Chen, Walter Verweij, Kamel Witek, Jonathan D.G. Jones, Ingo Hein

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher readdepth for selected genes and is therefore suitable for determination of sequence polymorphisms and enables multiplexing of samples. Target enrichment also provides a means to annotate specific gene families within the sequenced organisms without the requirements for gene models. Here we describe enrichment procedures for NB-LRR-type plant resistance genes that can, for example, be used to establish the NB-LRR gene complements of individual plants and to map resistances more rapidly using a bulked segregant analysis.

Original languageEnglish
Title of host publicationPlant-Pathogen Interactions
EditorsPaul Birch, John T. Jones, Jorunn I.B. Bos
PublisherSpringer
Pages291-303
Number of pages13
Volume1127
ISBN (Electronic)978-1-62703-986-4
ISBN (Print)978-1-62703-985-7
DOIs
Publication statusPublished - 15 Feb 2014

Publication series

NameMethods in Molecular Biology
PublisherSpringer Verlag
ISSN (Print)1064-3745

Keywords

  • Agilent SureSelect
  • High-throughput sequencing
  • NB-LRR genes
  • Target enrichment

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