@inbook{2df6bac579174c81bf0452c327bb6f5d,
title = "Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants",
abstract = "Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher readdepth for selected genes and is therefore suitable for determination of sequence polymorphisms and enables multiplexing of samples. Target enrichment also provides a means to annotate specific gene families within the sequenced organisms without the requirements for gene models. Here we describe enrichment procedures for NB-LRR-type plant resistance genes that can, for example, be used to establish the NB-LRR gene complements of individual plants and to map resistances more rapidly using a bulked segregant analysis.",
keywords = "Agilent SureSelect, High-throughput sequencing, NB-LRR genes, Target enrichment",
author = "Florian Jupe and Xinwei Chen and Walter Verweij and Kamel Witek and Jones, {Jonathan D.G.} and Ingo Hein",
year = "2014",
month = feb,
day = "15",
doi = "10.1007/978-1-62703-986-4_22",
language = "English",
isbn = "978-1-62703-985-7",
volume = "1127",
series = "Methods in Molecular Biology",
publisher = "Springer ",
pages = "291--303",
editor = "Birch, {Paul } and Jones, {John T. } and Bos, {Jorunn I.B. }",
booktitle = "Plant-Pathogen Interactions",
}