Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex

Ramon Hurtado-Guerrero, Olawale Raimi, Sharon Shepherd, Daan M. F. van Aalten

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    11 Citations (Scopus)

    Abstract

    Glucosamine-6-phosphate N-acetyltransferase (GNA1) catalyses the N-acetylation of D-glucosamine-6-phosphate (GlcN-6P), using acetyl-CoA as an acetyl donor. The product GlcNAc-6P is an intermediate in the biosynthesis UDP-GlcNAc. GNA1 is part of the GCN5-related acetyl transferase family (GNATs), which employ a wide range of acceptor substrates. GNA1 has been genetically validated as an antifungal drug target. Detailed knowledge of the Michaelis complex and trajectory towards the transition state would facilitate rational design of inhibitors of GNA1 and other GNAT enzymes. Using the pseudo-substrate glucose-6-phosphate (Glc-6P) as a probe with GNA1 crystals, we have trapped the first GNAT (pseudo-) Michaelis complex, providing direct evidence for the nucleophilic attack of the substrate amine, and giving insight into the protonation of the thiolate leaving group. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)5597-5600
    Number of pages4
    JournalFEBS Letters
    Volume581
    Issue number29
    DOIs
    Publication statusPublished - 11 Dec 2007

    Keywords

    • Crystal structure
    • Michaelis complex
    • Acetyltransferase
    • UDP-GlcNAc
    • Kinetics
    • Inhibitor
    • Blastocladiella emersonii
    • Hexosamine biosynthesis
    • Acetylglucosamine
    • Protein

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