Glutathione S-transferase in human bile

A F Howie, P C Hayes, I A Bouchier, J D Hayes, G J Beckett

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Glutathione S-transferase (GST) isoenzymes have been measured by specific radioimmunoassay in human bile samples. GST Mu was found in 50% of samples while GST Pi, GST B1 and GST B2 were present in all samples; GST Pi constituted the major isoenzyme identified. The findings of the radioimmunoassay were confirmed by a one-step purification of GST from bile, using affinity chromatography, followed by their identification using sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE). Inhibition studies showed that, at the concentrations of bile salts found in bile, GST Pi would have little or no enzymic activity. It is proposed that GST Pi acts as a carrier protein of toxic, non-substrate, ligands to remove as yet unidentified substances from biliary epithelial cells and prevent their reabsorption.

    Original languageEnglish
    Pages (from-to)269-78
    Number of pages10
    JournalClinica Chimica Acta
    Volume184
    Issue number3
    DOIs
    Publication statusPublished - 16 Oct 1989

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    Glutathione S-Transferase pi
    Glutathione Transferase
    Bile
    Isoenzymes
    Radioimmunoassay
    Affinity chromatography
    Poisons
    Bile Acids and Salts
    Sodium Dodecyl Sulfate
    Purification
    Carrier Proteins
    Affinity Chromatography
    Ligands
    Epithelial Cells

    Keywords

    • Bile/enzymology
    • Chromatography, Gel
    • Glutathione Transferase/antagonists & inhibitors
    • Humans
    • Isoenzymes/isolation & purification
    • Radioimmunoassay

    Cite this

    Howie, A. F., Hayes, P. C., Bouchier, I. A., Hayes, J. D., & Beckett, G. J. (1989). Glutathione S-transferase in human bile. Clinica Chimica Acta, 184(3), 269-78. https://doi.org/10.1016/0009-8981(89)90060-0
    Howie, A F ; Hayes, P C ; Bouchier, I A ; Hayes, J D ; Beckett, G J. / Glutathione S-transferase in human bile. In: Clinica Chimica Acta. 1989 ; Vol. 184, No. 3. pp. 269-78.
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    Howie, AF, Hayes, PC, Bouchier, IA, Hayes, JD & Beckett, GJ 1989, 'Glutathione S-transferase in human bile', Clinica Chimica Acta, vol. 184, no. 3, pp. 269-78. https://doi.org/10.1016/0009-8981(89)90060-0

    Glutathione S-transferase in human bile. / Howie, A F; Hayes, P C; Bouchier, I A; Hayes, J D; Beckett, G J.

    In: Clinica Chimica Acta, Vol. 184, No. 3, 16.10.1989, p. 269-78.

    Research output: Contribution to journalArticle

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    T1 - Glutathione S-transferase in human bile

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    AU - Hayes, P C

    AU - Bouchier, I A

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    Howie AF, Hayes PC, Bouchier IA, Hayes JD, Beckett GJ. Glutathione S-transferase in human bile. Clinica Chimica Acta. 1989 Oct 16;184(3):269-78. https://doi.org/10.1016/0009-8981(89)90060-0