Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles

J D Hayes; R E Coulthwaite; P K Stockman; A J Hussey; T J Mantle; C R Wolf

Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles

J D Hayes, R E Coulthwaite, P K Stockman, A J Hussey, T J Mantle, C R Wolf

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hexylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24,500)-, Ya(Mr 26,000)- and Yb(Mr 27,000)-type subunits. The isoelectric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8-8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.

Original language	English
Title of host publication	Mouse Liver Tumors
Pages	136-46
Number of pages	11
Volume	10
Publication status	Published - 1987
Event	Relevance to Human Cancer Risk Symposium of the European Society of Toxicology - Rome, Italy Duration: 2 Feb 1986 → 5 Feb 1986 https://link.springer.com/book/10.1007/978-3-642-71617-1

Publication series

Name	Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement
ISSN (Print)	0171-9750

Conference

Conference	Relevance to Human Cancer Risk Symposium of the European Society of Toxicology
Country/Territory	Italy
City	Rome
Period	2/02/86 → 5/02/86
Internet address	https://link.springer.com/book/10.1007/978-3-642-71617-1

Keywords

Animals
Chromatography
Cricetinae
Electrophoresis, Polyacrylamide Gel
Glutathione Transferase/isolation & purification
Guinea Pigs
Humans
Isoenzymes/isolation & purification
Liver/enzymology
Male
Mice
Rats
Species Specificity
Substrate Specificity
Tissue Distribution

Cite this

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title = "Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles",

abstract = "The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hexylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24,500)-, Ya(Mr 26,000)- and Yb(Mr 27,000)-type subunits. The isoelectric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8-8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.",

keywords = "Animals, Chromatography, Cricetinae, Electrophoresis, Polyacrylamide Gel, Glutathione Transferase/isolation & purification, Guinea Pigs, Humans, Isoenzymes/isolation & purification, Liver/enzymology, Male, Mice, Rats, Species Specificity, Substrate Specificity, Tissue Distribution",

author = "Hayes, {J D} and Coulthwaite, {R E} and Stockman, {P K} and Hussey, {A J} and Mantle, {T J} and Wolf, {C R}",

year = "1987",

language = "English",

volume = "10",

series = "Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement",

pages = "136--46",

booktitle = "Mouse Liver Tumors",

note = "Relevance to Human Cancer Risk Symposium of the European Society of Toxicology ; Conference date: 02-02-1986 Through 05-02-1986",

url = "https://link.springer.com/book/10.1007/978-3-642-71617-1",

}

Hayes, JD, Coulthwaite, RE, Stockman, PK, Hussey, AJ, Mantle, TJ & Wolf, CR 1987, Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles. in Mouse Liver Tumors. vol. 10, Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement, pp. 136-46, Relevance to Human Cancer Risk Symposium of the European Society of Toxicology, Rome, Italy, 2/02/86.

Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles. / Hayes, J D; Coulthwaite, R E; Stockman, P K et al.
Mouse Liver Tumors. Vol. 10 1987. p. 136-46 (Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles

AU - Hayes, J D

AU - Coulthwaite, R E

AU - Stockman, P K

AU - Hussey, A J

AU - Mantle, T J

AU - Wolf, C R

PY - 1987

Y1 - 1987

N2 - The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hexylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24,500)-, Ya(Mr 26,000)- and Yb(Mr 27,000)-type subunits. The isoelectric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8-8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.

AB - The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hexylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24,500)-, Ya(Mr 26,000)- and Yb(Mr 27,000)-type subunits. The isoelectric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8-8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.

KW - Animals

KW - Chromatography

KW - Cricetinae

KW - Electrophoresis, Polyacrylamide Gel

KW - Glutathione Transferase/isolation & purification

KW - Guinea Pigs

KW - Humans

KW - Isoenzymes/isolation & purification

KW - Liver/enzymology

KW - Male

KW - Mice

KW - Rats

KW - Species Specificity

KW - Substrate Specificity

KW - Tissue Distribution

UR - https://link.springer.com/chapter/10.1007/978-3-642-71617-1_11

M3 - Conference contribution

C2 - 3472496

VL - 10

T3 - Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement

SP - 136

EP - 146

BT - Mouse Liver Tumors

T2 - Relevance to Human Cancer Risk Symposium of the European Society of Toxicology

Y2 - 2 February 1986 through 5 February 1986

ER -

Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles

Abstract

Publication series

Conference

Keywords

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Cite this