Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles

J D Hayes, R E Coulthwaite, P K Stockman, A J Hussey, T J Mantle, C R Wolf

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    17 Citations (Scopus)

    Abstract

    The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hexylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24,500)-, Ya(Mr 26,000)- and Yb(Mr 27,000)-type subunits. The isoelectric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8-8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.

    Original languageEnglish
    Title of host publicationMouse Liver Tumors
    Pages136-46
    Number of pages11
    Volume10
    Publication statusPublished - 1987
    EventRelevance to Human Cancer Risk Symposium of the European Society of Toxicology - Rome, Italy
    Duration: 2 Feb 19865 Feb 1986
    https://link.springer.com/book/10.1007/978-3-642-71617-1

    Publication series

    NameArchives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement
    ISSN (Print)0171-9750

    Conference

    ConferenceRelevance to Human Cancer Risk Symposium of the European Society of Toxicology
    CountryItaly
    CityRome
    Period2/02/865/02/86
    Internet address

    Keywords

    • Animals
    • Chromatography
    • Cricetinae
    • Electrophoresis, Polyacrylamide Gel
    • Glutathione Transferase/isolation & purification
    • Guinea Pigs
    • Humans
    • Isoenzymes/isolation & purification
    • Liver/enzymology
    • Male
    • Mice
    • Rats
    • Species Specificity
    • Substrate Specificity
    • Tissue Distribution

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  • Cite this

    Hayes, J. D., Coulthwaite, R. E., Stockman, P. K., Hussey, A. J., Mantle, T. J., & Wolf, C. R. (1987). Glutathione S-transferase subunits in the mouse and their catalytic activities towards reactive electrophiles. In Mouse Liver Tumors (Vol. 10, pp. 136-46). (Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement).