Glutathionylspermidine metabolism in Escherichia coli

TY - JOUR

T1 - Glutathionylspermidine metabolism in Escherichia coli

AU - Smith, K.

AU - Borges, A.

AU - Ariyanayagam, M. R.

AU - Fairlamb, A. H.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - Intracellular levels of glutathione and glutathionylspermidine conjugates have been measured throughout the growth phases of Escherichia coli. Glutathionylspermidine was present in mid-log-phase cells, and under stationary and anaerobic growth conditions accounted for 80% of the total glutathione content. N1,N8-bis(glutathionyl)spermidine (trypanothione) was undetectable under all growth conditions. The catalytic constant k(cat.)/K(m)) of recombinant E. coli glutathione reductase for glutathionylspermidine disulphide was approx. 11000-fold lower than that for glutathione disulphide. The much higher catalytic constant for the mixed disulphide of glutathione and glutathionylspermidine (11% that of GSSG), suggests a possible explanation for the low turnover of trypanothione disulphide by E. coli glutathione reductase, given the apparent lack of a specific glutathionylspermidine disulphide reductase in E. coli.

AB - Intracellular levels of glutathione and glutathionylspermidine conjugates have been measured throughout the growth phases of Escherichia coli. Glutathionylspermidine was present in mid-log-phase cells, and under stationary and anaerobic growth conditions accounted for 80% of the total glutathione content. N1,N8-bis(glutathionyl)spermidine (trypanothione) was undetectable under all growth conditions. The catalytic constant k(cat.)/K(m)) of recombinant E. coli glutathione reductase for glutathionylspermidine disulphide was approx. 11000-fold lower than that for glutathione disulphide. The much higher catalytic constant for the mixed disulphide of glutathione and glutathionylspermidine (11% that of GSSG), suggests a possible explanation for the low turnover of trypanothione disulphide by E. coli glutathione reductase, given the apparent lack of a specific glutathionylspermidine disulphide reductase in E. coli.

UR - https://www.scopus.com/pages/publications/0029586070

U2 - 10.1042/bj3120465

DO - 10.1042/bj3120465

M3 - Article

C2 - 8526857

AN - SCOPUS:0029586070

SN - 0264-6021

VL - 312

SP - 465

EP - 469

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -