Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco

David C. Gershlick, Carine De Marcos Lousa, Ombretta Foresti, Andrew J. Lee, Estela A. Pereira, Luis L. P. daSilva, Francesca Bottanelli, Jurgen Denecke

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The cycling of vacuolar sorting receptors (VSRs) between early and late secretory pathway compartments is regulated by signals in the cytosolic tail, but the exact pathway is controversial. Here, we show that receptor targeting in tobacco (Nicotiana tabacum) initially involves a canonical coat protein complex II-dependent endoplasmic reticulum-to-Golgi bulk flow route and that VSR-ligand interactions in the cis-Golgi play an important role in vacuolar sorting. We also show that a conserved Glu is required but not sufficient for rate-limiting YXXΦ{phonetic}-mediated receptor trafficking. Protein-protein interaction studies show that the VSR tail interacts with the μ-subunits of plant or mammalian clathrin adaptor complex AP1 and plant AP4 but not that of plant and mammalian AP2. Mutants causing a detour of full-length receptors via the cell surface invariantly cause the secretion of VSR ligands. Therefore, we propose that cycling via the plasma membrane is unlikely to play a role in biosynthetic vacuolar sorting under normal physiological conditions and that the conserved Ile-Met motif is mainly used to recover mistargeted receptors. This occurs via a fundamentally different pathway from the prevacuolar compartment that does not mediate recycling. The role of clathrin and clathrin-independent pathways in vacuolar targeting is discussed.

Original languageEnglish
Pages (from-to)1308-1329
Number of pages22
JournalPlant Cell
Volume26
Issue number3
Early online date18 Mar 2014
DOIs
Publication statusPublished - May 2014

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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