GPIomics: global analysis of glycosylphosphatidylinositol-anchored molecules of Trypanosoma cruzi

Ernesto S. Nakayasu, Dmitry V. Yashunsky, Lilian L. Nohara, Ana Claudia T. Torrecilhas, Andrei V. Nikolaev, Igor C. Almeida (Lead / Corresponding author)

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    Abstract

    Glycosylphosphatidylinositol (GPI) anchoring is a common, relevant posttranslational modification of eukaryotic surface proteins. Here, we developed a fast, simple, and highly sensitive (high attomole-low femtomole range) method that uses liquid chromatography-tandem mass spectrometry (LC-MSn) for the first large-scale analysis of GPI-anchored molecules (i.e., the GPIome) of a eukaryote, Trypanosoma cruzi, the etiologic agent of Chagas disease. Our genome-wise prediction analysis revealed that approximately 12% of T. cruzi genes possibly encode GPI-anchored proteins. By analyzing the GPIome of T. cruzi insect-dwelling epimastigote stage using LC-MSn, we identified 90 GPI species, of which 79 were novel. Moreover, we determined that mucins coded by the T. cruzi small mucin-like gene (TcSMUG S) family are the major GPI-anchored proteins expressed on the epimastigote cell surface. TcSMUG S mucin mature sequences are short (56-85 amino acids) and highly O-glycosylated, and contain few proteolytic sites, therefore, less likely susceptible to proteases of the midgut of the insect vector. We propose that our approach could be used for the high throughput GPIomic analysis of other lower and higher eukaryotes. Molecular Systems Biology 7 April 2009; doi:10.1038/msb.2009.13

    Original languageEnglish
    Article number261
    Number of pages17
    JournalMolecular Systems Biology
    Volume5
    DOIs
    Publication statusPublished - Apr 2009

    Keywords

    • global analysis
    • glycobiology
    • glycosylphosphatidylinositol
    • GPIomics
    • protein posttranslational modifications
    • CHAGAS-DISEASE
    • MASS-SPECTROMETRY
    • STRUCTURAL-CHARACTERIZATION
    • PROTEIN IDENTIFICATION
    • AMERICAN TRYPANOSOMIASIS
    • ELECTROSPRAY-IONIZATION
    • TRYPOMASTIGOTE FORMS
    • EPIMASTIGOTE FORMS
    • ESCHERICHIA-COLI
    • LATIN-AMERICA

    Cite this

    Nakayasu, E. S., Yashunsky, D. V., Nohara, L. L., Torrecilhas, A. C. T., Nikolaev, A. V., & Almeida, I. C. (2009). GPIomics: global analysis of glycosylphosphatidylinositol-anchored molecules of Trypanosoma cruzi. Molecular Systems Biology, 5, [261]. https://doi.org/10.1038/msb.2009.13