Growth of large and highly ordered 2D crystals of a K+ channel, structural role of lipidic environment

Rita De Zorzi; William V. Nicholson; Jean-Michel Guigner; Françoise Erne-Brand; Catherine Vénien-Bryan

doi:10.1016/j.bpj.2013.05.054

Growth of large and highly ordered 2D crystals of a K⁺ channel, structural role of lipidic environment

Rita De Zorzi, William V. Nicholson, Jean-Michel Guigner, Françoise Erne-Brand, Catherine Vénien-Bryan

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

2D crystallography has proven to be an excellent technique to determine the 3D structure of membrane proteins. Compared to 3D crystallography, it has the advantage of visualizing the protein in an environment closer to the native one. However, producing good 2D crystals is still a challenge and little statistical knowledge can be gained from literature. Here, we present a thorough screening of 2D crystallization conditions for a prokaryotic inwardly rectifying potassium channel (>130 different conditions). Key parameters leading to very large and well-organized 2D crystals are discussed. In addition, the problem of formation of multilayers during the growth of 2D crystals is also addressed. An intermediate resolution projection map of KirBac3.1 at 6 Å is presented, which sheds (to our knowledge) new light on the structure of this channel in a lipid environment.

Original language	English
Pages (from-to)	398-408
Number of pages	11
Journal	Biophysical Journal
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/j.bpj.2013.05.054
Publication status	Published - 16 Jul 2013

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10.1016/j.bpj.2013.05.054

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title = "Growth of large and highly ordered 2D crystals of a K+ channel, structural role of lipidic environment",

abstract = "2D crystallography has proven to be an excellent technique to determine the 3D structure of membrane proteins. Compared to 3D crystallography, it has the advantage of visualizing the protein in an environment closer to the native one. However, producing good 2D crystals is still a challenge and little statistical knowledge can be gained from literature. Here, we present a thorough screening of 2D crystallization conditions for a prokaryotic inwardly rectifying potassium channel (>130 different conditions). Key parameters leading to very large and well-organized 2D crystals are discussed. In addition, the problem of formation of multilayers during the growth of 2D crystals is also addressed. An intermediate resolution projection map of KirBac3.1 at 6 {\AA} is presented, which sheds (to our knowledge) new light on the structure of this channel in a lipid environment.",

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AU - De Zorzi, Rita

AU - Nicholson, William V.

AU - Guigner, Jean-Michel

AU - Erne-Brand, Françoise

AU - Vénien-Bryan, Catherine

PY - 2013/7/16

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AB - 2D crystallography has proven to be an excellent technique to determine the 3D structure of membrane proteins. Compared to 3D crystallography, it has the advantage of visualizing the protein in an environment closer to the native one. However, producing good 2D crystals is still a challenge and little statistical knowledge can be gained from literature. Here, we present a thorough screening of 2D crystallization conditions for a prokaryotic inwardly rectifying potassium channel (>130 different conditions). Key parameters leading to very large and well-organized 2D crystals are discussed. In addition, the problem of formation of multilayers during the growth of 2D crystals is also addressed. An intermediate resolution projection map of KirBac3.1 at 6 Å is presented, which sheds (to our knowledge) new light on the structure of this channel in a lipid environment.

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DO - 10.1016/j.bpj.2013.05.054

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Growth of large and highly ordered 2D crystals of a K⁺ channel, structural role of lipidic environment

Abstract

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Erratum: Growth of large and highly ordered 2D crystals of a KD channel, structural role of lipidic environment (Biophysical Journal (2013) 105: 2 (398-408))

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