H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex

Giulia Saredi, Hongda Huang, Colin M. Hammond, Constance Alabert, Simon Bekker-Jensen, Ignasi Forne, Nazaret Reverón-Gómez, Benjamin M. Foster, Lucie Mlejnkova, Till Bartke, Petr Cejka, Niels Mailand, Axel Imhof, Dinshaw J. Patel (Lead / Corresponding author), Anja Groth (Lead / Corresponding author)

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

After DNA replication, chromosomal processes including DNA repair and transcription take place in the context of sister chromatids. While cell cycle regulation can guide these processes globally, mechanisms to distinguish pre- and post-replicative states locally remain unknown. Here we reveal that new histones incorporated during DNA replication provide a signature of postreplicative chromatin, read by the human TONSL-MMS22L1-4 homologous recombination complex. We identify the TONSL ankyrin repeat domain (ARD) as a reader of histone H4 tails unmethylated at K20 (H4K20me0), which are specific to new histones incorporated during DNA replication and mark postreplicative chromatin until the G2/M phase of the cell cycle. Accordingly, TONSL-MMS22L binds new histones H3-H4 both before and after incorporation into nucleosomes, remaining on replicated chromatin until late G2/M. H4K20me0 recognition is required for TONSL-MMS22L binding to chromatin and accumulation at challenged replication forks and DNA lesions. Consequently, TONSL ARD mutants are toxic, compromising genome stability, cell viability and resistance to replication stress. Together, these data reveal a histone-reader-based mechanism for recognizing the post-replicative state, offering a new angle to understand DNA repair with the potential for targeted cancer therapy.

Original languageEnglish
Pages (from-to)714-718
Number of pages5
JournalNature
Volume534
Issue number7609
DOIs
Publication statusPublished - 22 Jun 2016

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DNA Repair
Histones
Chromatin
DNA Replication
Ankyrin Repeat
Cell Cycle
Chromatids
Nucleosomes
Genomic Instability
G2 Phase
Poisons
Homologous Recombination
Cell Division
Tail
Cell Survival
Neoplasms

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Saredi, G., Huang, H., Hammond, C. M., Alabert, C., Bekker-Jensen, S., Forne, I., ... Groth, A. (2016). H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex. Nature, 534(7609), 714-718. https://doi.org/10.1038/nature18312
Saredi, Giulia ; Huang, Hongda ; Hammond, Colin M. ; Alabert, Constance ; Bekker-Jensen, Simon ; Forne, Ignasi ; Reverón-Gómez, Nazaret ; Foster, Benjamin M. ; Mlejnkova, Lucie ; Bartke, Till ; Cejka, Petr ; Mailand, Niels ; Imhof, Axel ; Patel, Dinshaw J. ; Groth, Anja. / H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex. In: Nature. 2016 ; Vol. 534, No. 7609. pp. 714-718.
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Saredi, G, Huang, H, Hammond, CM, Alabert, C, Bekker-Jensen, S, Forne, I, Reverón-Gómez, N, Foster, BM, Mlejnkova, L, Bartke, T, Cejka, P, Mailand, N, Imhof, A, Patel, DJ & Groth, A 2016, 'H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex', Nature, vol. 534, no. 7609, pp. 714-718. https://doi.org/10.1038/nature18312

H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex. / Saredi, Giulia; Huang, Hongda; Hammond, Colin M.; Alabert, Constance; Bekker-Jensen, Simon; Forne, Ignasi; Reverón-Gómez, Nazaret; Foster, Benjamin M.; Mlejnkova, Lucie; Bartke, Till; Cejka, Petr; Mailand, Niels; Imhof, Axel; Patel, Dinshaw J. (Lead / Corresponding author); Groth, Anja (Lead / Corresponding author).

In: Nature, Vol. 534, No. 7609, 22.06.2016, p. 714-718.

Research output: Contribution to journalArticle

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T1 - H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex

AU - Saredi, Giulia

AU - Huang, Hongda

AU - Hammond, Colin M.

AU - Alabert, Constance

AU - Bekker-Jensen, Simon

AU - Forne, Ignasi

AU - Reverón-Gómez, Nazaret

AU - Foster, Benjamin M.

AU - Mlejnkova, Lucie

AU - Bartke, Till

AU - Cejka, Petr

AU - Mailand, Niels

AU - Imhof, Axel

AU - Patel, Dinshaw J.

AU - Groth, Anja

PY - 2016/6/22

Y1 - 2016/6/22

N2 - After DNA replication, chromosomal processes including DNA repair and transcription take place in the context of sister chromatids. While cell cycle regulation can guide these processes globally, mechanisms to distinguish pre- and post-replicative states locally remain unknown. Here we reveal that new histones incorporated during DNA replication provide a signature of postreplicative chromatin, read by the human TONSL-MMS22L1-4 homologous recombination complex. We identify the TONSL ankyrin repeat domain (ARD) as a reader of histone H4 tails unmethylated at K20 (H4K20me0), which are specific to new histones incorporated during DNA replication and mark postreplicative chromatin until the G2/M phase of the cell cycle. Accordingly, TONSL-MMS22L binds new histones H3-H4 both before and after incorporation into nucleosomes, remaining on replicated chromatin until late G2/M. H4K20me0 recognition is required for TONSL-MMS22L binding to chromatin and accumulation at challenged replication forks and DNA lesions. Consequently, TONSL ARD mutants are toxic, compromising genome stability, cell viability and resistance to replication stress. Together, these data reveal a histone-reader-based mechanism for recognizing the post-replicative state, offering a new angle to understand DNA repair with the potential for targeted cancer therapy.

AB - After DNA replication, chromosomal processes including DNA repair and transcription take place in the context of sister chromatids. While cell cycle regulation can guide these processes globally, mechanisms to distinguish pre- and post-replicative states locally remain unknown. Here we reveal that new histones incorporated during DNA replication provide a signature of postreplicative chromatin, read by the human TONSL-MMS22L1-4 homologous recombination complex. We identify the TONSL ankyrin repeat domain (ARD) as a reader of histone H4 tails unmethylated at K20 (H4K20me0), which are specific to new histones incorporated during DNA replication and mark postreplicative chromatin until the G2/M phase of the cell cycle. Accordingly, TONSL-MMS22L binds new histones H3-H4 both before and after incorporation into nucleosomes, remaining on replicated chromatin until late G2/M. H4K20me0 recognition is required for TONSL-MMS22L binding to chromatin and accumulation at challenged replication forks and DNA lesions. Consequently, TONSL ARD mutants are toxic, compromising genome stability, cell viability and resistance to replication stress. Together, these data reveal a histone-reader-based mechanism for recognizing the post-replicative state, offering a new angle to understand DNA repair with the potential for targeted cancer therapy.

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