Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection

G. Tacconi, V. Baldassarre, N. C. Collins, D. Bulgarelli, A. M. Stanca, G. Valè

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, weconducted a polymorphism search in 3 predicted low-copy sequence regions adjacent to the Mlo gene by examining a sample of 4 Mlo and 3 mlo cultivars. Eight single-nucleotide polymorphisms (SNPs) and 1 insertion-deletion (indel) were detected, and easy to use PGR-based markers were developed for typing the SNPs. The PCR markers were used to characterize a collection of 46 Mlo and 25 mlo barley cultivars, identifying 3 distinct mlo-11 haplotypes, 1 mlo-9 haplotype, and 4 Mlo haplotypes. We summarized the haplotype and marker information obtained here and in a previous study to help breeders identify strategies for mlo marker-assisted selection. The ability of the markers to identify mlo-resistant genotypes in segregating populations was demonstrated using 2 resistance-characterized F2 populations derived by 3-way crosses.

Original languageEnglish
Pages (from-to)864-872
Number of pages9
JournalGenome
Volume49
Issue number8
DOIs
Publication statusPublished - 1 Aug 2006

Keywords

  • Barley
  • Marker-assisted selection
  • Mlo
  • Powdery mildew resistance
  • SNPs

Fingerprint Dive into the research topics of 'Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection'. Together they form a unique fingerprint.

Cite this