HDM2 negatively affects the Chk2-mediated phosphorylation of p53

Marina Bjørling-Poulsen, David Meek, Olaf-Georg Issinger

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    2 Citations (Scopus)

    Abstract

    By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.
    Original languageEnglish
    Pages (from-to)2604-2608
    Number of pages5
    JournalFEBS Letters
    Volume579
    Issue number12
    DOIs
    Publication statusPublished - 9 May 2005

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    Bjørling-Poulsen, M., Meek, D., & Issinger, O-G. (2005). HDM2 negatively affects the Chk2-mediated phosphorylation of p53. FEBS Letters, 579(12), 2604-2608. https://doi.org/10.1016/j.febslet.2005.03.081