TY - JOUR
T1 - HDM2 negatively affects the Chk2-mediated phosphorylation of p53
AU - Bjørling-Poulsen, Marina
AU - Meek, David
AU - Issinger, Olaf-Georg
PY - 2005/5/9
Y1 - 2005/5/9
N2 - By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.
AB - By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.
U2 - 10.1016/j.febslet.2005.03.081
DO - 10.1016/j.febslet.2005.03.081
M3 - Article
C2 - 15862297
SN - 0014-5793
VL - 579
SP - 2604
EP - 2608
JO - FEBS Letters
JF - FEBS Letters
IS - 12
ER -