HDM2 negatively affects the Chk2-mediated phosphorylation of p53

Marina Bjørling-Poulsen; David Meek; Olaf-Georg Issinger

doi:10.1016/j.febslet.2005.03.081

HDM2 negatively affects the Chk2-mediated phosphorylation of p53

Marina Bjørling-Poulsen
, David Meek
, Olaf-Georg Issinger

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.

Original language	English
Pages (from-to)	2604-2608
Number of pages	5
Journal	FEBS Letters
Volume	579
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2005.03.081
Publication status	Published - 9 May 2005

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title = "HDM2 negatively affects the Chk2-mediated phosphorylation of p53",

abstract = "By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.",

author = "Marina Bj{\o}rling-Poulsen and David Meek and Olaf-Georg Issinger",

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T1 - HDM2 negatively affects the Chk2-mediated phosphorylation of p53

AU - Bjørling-Poulsen, Marina

AU - Meek, David

AU - Issinger, Olaf-Georg

PY - 2005/5/9

Y1 - 2005/5/9

N2 - By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.

AB - By GST pull downs and co-immunoprecipitation analyses we found that recombinant Chk2 and HDM2 can form stable complexes in vitro. Chk2/HDM2 complexes were also detected in transfected Cos-1 cells over-expressing both proteins. Furthermore, we show that HDM2, as would be expected, severely affects the Chk2-catalyzed phosphorylation of p53. HDM2 itself is only slightly phosphorylated by Chk2. However, whereas HDM2 inhibits the Chk2-catalyzed p53 phosphorylation, HDM2 phosphorylation by Chk2 doubles in the presence of p53. The significance of the HDM2 phosphorylation is unknown, but it is possible that it might influence the stability of the HDM2/p53 complex.

U2 - 10.1016/j.febslet.2005.03.081

DO - 10.1016/j.febslet.2005.03.081

M3 - Article

C2 - 15862297

SN - 0014-5793

VL - 579

SP - 2604

EP - 2608

JO - FEBS Letters

JF - FEBS Letters

IS - 12

ER -

HDM2 negatively affects the Chk2-mediated phosphorylation of p53

Abstract

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