Heavy metal uptake by intact cells and protoplasts of Aureobasidium pullulans

G. M. Gadd, C. White, J. L. Mowll

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    Protoplasts prepared from yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans can take up heavy metals such as Zn2+, Co2+, Cd2+ and Cu2+. In relation to intact cells, the sensitivity of protoplasts to Cu2+ and Cd2+ was increased although chlamydospore protoplasts were more tolerant than yeast-like cell protoplasts. Surface binding of metals was reduced in protoplasts as compared with intact cells and this reduction was particularly evident for chlamydospore protoplasts. At the highest concentrations used, uptake of Zn2+, Co2+ and Cd2+ by yeast-like cell protoplasts was greater than that observed in intact cells which may have been due to toxicity, especially for Cd2+, resulting in increased membrane permeability, though for Zn2+ and Co2+ some barrier effect of the cell wall could not be completely discounted. Chlamydospore protoplasts were capable of intracellular metal uptake, unlike intact chlamydospores, and for Zn2+, uptake appeared to be via a different system less specific than that of the other cell types. For chlamydospores, the use of protoplasts confirmed the importance of the cell wall in preventing entry of metal ions into the cell.

    Original languageEnglish
    Pages (from-to)261-267
    Number of pages7
    JournalFEMS Microbiology Letters
    Volume45
    Issue number5
    DOIs
    Publication statusPublished - Oct 1987

    Keywords

    • Aureobasidium pullulans
    • Heavy metals
    • Protoplasts

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