Hepatic damage in the rat following administration of thyroxine or triiodothyronine, assessed by measurement of plasma glutathione S-transferase YaYa concentrations

G J Beckett, J E Hunter, J D Hayes

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    Abstract

    A specific radioimmunoassay (RIA) has been developed that has sufficient sensitivity to allow measurement of the changes in plasma and tissue glutathione S-transferase (GST) YaYa concentrations which occur following thyroid hormone administration in the rat. Using the RIA it was demonstrated that the only tissues that had significant amounts of GST YaYa were liver, small gut and kidney. Administration of triiodothyronine (T3) or thyroxine (T4) resulted in increases in plasma GST YaYa concentration and in animals given high doses of T4 plasma alanine aminotransferase activity was also elevated. Thyroid hormone administration produced a significant fall in the hepatic content of GST YaYa and in total GST activity, as assessed using 1-chloro-2,4-dinitrobenzene as substrate. It is concluded that the elevated plasma GST YaYa concentrations observed following administration of thyroid hormones result from hepatic damage, not from induction of hepatic synthesis of the enzyme.

    Original languageEnglish
    Pages (from-to)69-79
    Number of pages11
    JournalClinica Chimica Acta
    Volume161
    Issue number1
    DOIs
    Publication statusPublished - 30 Nov 1986

    Fingerprint

    Triiodothyronine
    Glutathione Transferase
    Thyroxine
    Rats
    Plasmas
    Liver
    Thyroid Hormones
    Radioimmunoassay
    Tissue
    Dinitrochlorobenzene
    Alanine Transaminase
    Animals
    Kidney
    Substrates
    Enzymes

    Keywords

    • Animals
    • Cytosol/drug effects
    • Glutathione Transferase/blood
    • Isoenzymes/blood
    • Liver/drug effects
    • Male
    • Radioimmunoassay
    • Rats
    • Rats, Inbred Strains
    • Thyroxine/blood
    • Triiodothyronine/blood

    Cite this

    @article{9742e37e7dd7485b8b4ac52ad29fd127,
    title = "Hepatic damage in the rat following administration of thyroxine or triiodothyronine, assessed by measurement of plasma glutathione S-transferase YaYa concentrations",
    abstract = "A specific radioimmunoassay (RIA) has been developed that has sufficient sensitivity to allow measurement of the changes in plasma and tissue glutathione S-transferase (GST) YaYa concentrations which occur following thyroid hormone administration in the rat. Using the RIA it was demonstrated that the only tissues that had significant amounts of GST YaYa were liver, small gut and kidney. Administration of triiodothyronine (T3) or thyroxine (T4) resulted in increases in plasma GST YaYa concentration and in animals given high doses of T4 plasma alanine aminotransferase activity was also elevated. Thyroid hormone administration produced a significant fall in the hepatic content of GST YaYa and in total GST activity, as assessed using 1-chloro-2,4-dinitrobenzene as substrate. It is concluded that the elevated plasma GST YaYa concentrations observed following administration of thyroid hormones result from hepatic damage, not from induction of hepatic synthesis of the enzyme.",
    keywords = "Animals, Cytosol/drug effects, Glutathione Transferase/blood, Isoenzymes/blood, Liver/drug effects, Male, Radioimmunoassay, Rats, Rats, Inbred Strains, Thyroxine/blood, Triiodothyronine/blood",
    author = "Beckett, {G J} and Hunter, {J E} and Hayes, {J D}",
    year = "1986",
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    doi = "10.1016/0009-8981(86)90264-0",
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    TY - JOUR

    T1 - Hepatic damage in the rat following administration of thyroxine or triiodothyronine, assessed by measurement of plasma glutathione S-transferase YaYa concentrations

    AU - Beckett, G J

    AU - Hunter, J E

    AU - Hayes, J D

    PY - 1986/11/30

    Y1 - 1986/11/30

    N2 - A specific radioimmunoassay (RIA) has been developed that has sufficient sensitivity to allow measurement of the changes in plasma and tissue glutathione S-transferase (GST) YaYa concentrations which occur following thyroid hormone administration in the rat. Using the RIA it was demonstrated that the only tissues that had significant amounts of GST YaYa were liver, small gut and kidney. Administration of triiodothyronine (T3) or thyroxine (T4) resulted in increases in plasma GST YaYa concentration and in animals given high doses of T4 plasma alanine aminotransferase activity was also elevated. Thyroid hormone administration produced a significant fall in the hepatic content of GST YaYa and in total GST activity, as assessed using 1-chloro-2,4-dinitrobenzene as substrate. It is concluded that the elevated plasma GST YaYa concentrations observed following administration of thyroid hormones result from hepatic damage, not from induction of hepatic synthesis of the enzyme.

    AB - A specific radioimmunoassay (RIA) has been developed that has sufficient sensitivity to allow measurement of the changes in plasma and tissue glutathione S-transferase (GST) YaYa concentrations which occur following thyroid hormone administration in the rat. Using the RIA it was demonstrated that the only tissues that had significant amounts of GST YaYa were liver, small gut and kidney. Administration of triiodothyronine (T3) or thyroxine (T4) resulted in increases in plasma GST YaYa concentration and in animals given high doses of T4 plasma alanine aminotransferase activity was also elevated. Thyroid hormone administration produced a significant fall in the hepatic content of GST YaYa and in total GST activity, as assessed using 1-chloro-2,4-dinitrobenzene as substrate. It is concluded that the elevated plasma GST YaYa concentrations observed following administration of thyroid hormones result from hepatic damage, not from induction of hepatic synthesis of the enzyme.

    KW - Animals

    KW - Cytosol/drug effects

    KW - Glutathione Transferase/blood

    KW - Isoenzymes/blood

    KW - Liver/drug effects

    KW - Male

    KW - Radioimmunoassay

    KW - Rats

    KW - Rats, Inbred Strains

    KW - Thyroxine/blood

    KW - Triiodothyronine/blood

    U2 - 10.1016/0009-8981(86)90264-0

    DO - 10.1016/0009-8981(86)90264-0

    M3 - Article

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    JO - Clinica Chimica Acta

    JF - Clinica Chimica Acta

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    ER -