Heterogeneity of protein kinase C expression and regulation in T lymphocytes

S. Lucas, R. Marais, J. D. Graves, D. Alexandere, P. Parker, D. A. Cantrell (Lead / Corresponding author)

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC α,β1, and β2 and γ, it was observed that T cells expressed two PKC isotypes, PKC α and β. No PKC γ was detected in T lymphocytes. In lymphoblasts, high levels of PKC β compared to PKC α were found whereas Jurkat cells expressed high levels of α compared to PKC β. Differences in the calcium sensitivity of phorbol ester-induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC α and β isotypes expressed by the cells.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalFEBS Letters
Volume260
Issue number1
DOIs
Publication statusPublished - 15 Jan 1990

    Fingerprint

Keywords

  • CD3 antigen
  • Protein kinase C
  • Signal transduction

Cite this