Heterogeneity of protein kinase C expression and regulation in T lymphocytes

TY - JOUR

T1 - Heterogeneity of protein kinase C expression and regulation in T lymphocytes

AU - Lucas, S.

AU - Marais, R.

AU - Graves, J. D.

AU - Alexandere, D.

AU - Parker, P.

AU - Cantrell, D. A.

PY - 1990/1/15

Y1 - 1990/1/15

N2 - The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC α,β1, and β2 and γ, it was observed that T cells expressed two PKC isotypes, PKC α and β. No PKC γ was detected in T lymphocytes. In lymphoblasts, high levels of PKC β compared to PKC α were found whereas Jurkat cells expressed high levels of α compared to PKC β. Differences in the calcium sensitivity of phorbol ester-induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC α and β isotypes expressed by the cells.

AB - The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC α,β1, and β2 and γ, it was observed that T cells expressed two PKC isotypes, PKC α and β. No PKC γ was detected in T lymphocytes. In lymphoblasts, high levels of PKC β compared to PKC α were found whereas Jurkat cells expressed high levels of α compared to PKC β. Differences in the calcium sensitivity of phorbol ester-induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC α and β isotypes expressed by the cells.

KW - CD3 antigen

KW - Protein kinase C

KW - Signal transduction

UR - https://www.scopus.com/pages/publications/0025128887

U2 - 10.1016/0014-5793(90)80064-P

DO - 10.1016/0014-5793(90)80064-P

M3 - Article

C2 - 2137095

AN - SCOPUS:0025128887

SN - 0014-5793

VL - 260

SP - 53

EP - 56

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -