High CCND1 amplification identifies a group of poor prognosis women with estrogen receptor positive breast cancer

Pankaj G. Roy, Norman Pratt, Colin A. Purdie, Lee Baker, Alison Ashfield, Phil Quinlan, Alastair M. Thompson

    Research output: Contribution to journalArticlepeer-review

    77 Citations (Scopus)

    Abstract

    CCND1 encodes for the cyclin D1 protein involved in G1/S cell cycle transition. In breast cancer the mechanism of CCND1 amplification, relationship between cyclin D1 protein expression and the key clinical markers estrogen receptor (ER) and HER2 requires elucidation. Tissue microarrays of primary invasive breast cancer from 93 women were evaluated for CCND1 amplification by fluorescent in-situ hybridization and cyclin D1 protein overexpression by immunohistochemistry. CCND1 amplification was identified in 27/93 (30%) cancers and 59/93 (63%) cancers had overexpression of cyclin D1. CCND1 amplification was significantly associated with cyclin D1 protein overexpression (p < 0.001; Fisher's exact test) and both CCND1 amplification and cyclin D1 protein expression with oestrogen receptor (ER) expression (p = 0.003 and p < 0.001; Fishers exact test). Neither CCND1 amplification nor cyclinD1 expression was associated with tumor size, pathological node status or HER2 amplification, but high CCND1 amplification (Copy Number Gain (CNG) >= 8) was associated with high tumor grade (p = 0.005; chi square 7.915, 2 df) and worse prognosis by Nottingham Prognostic Index (p = 0.001; 2 sample t-test). High CCND1 amplification (CNG >= 8) may identify a subset of patients with poor prognosis ER-positive breast cancers who should be considered for additional therapy.

    Original languageEnglish
    Pages (from-to)355-360
    Number of pages6
    JournalInternational Journal of Cancer
    Volume127
    Issue number2
    DOIs
    Publication statusPublished - 15 Jul 2010

    Keywords

    • breast cancer
    • cyclin D
    • amplification
    • HER2
    • prognosis
    • HOMOGENEOUSLY STAINING REGIONS
    • CYCLIN D1
    • GENE AMPLIFICATION
    • DOUBLE MINUTES
    • CELL-CYCLE
    • TAMOXIFEN RESISTANCE
    • TUMORS
    • TRASTUZUMAB
    • INSTABILITY
    • VALIDATION

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