High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

Sergey Turtaev, Ivo T. Leite, Tristan Altwegg-Boussac, Janelle M. P. Pakan, Nathalie L. Rochefort (Lead / Corresponding author), Tomáš Čižmár (Lead / Corresponding author)

Research output: Contribution to journalArticle

11 Citations (Scopus)
51 Downloads (Pure)

Abstract

Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 ± 0.04 µm across a 50-µm field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.

Original languageEnglish
Article number92
Pages (from-to)1-8
Number of pages8
JournalLight: Science and Applications
Volume7
DOIs
Publication statusPublished - 21 Nov 2018

Fingerprint

Endoscopy
Multimode fibers
brain
Brain
hippocampus
neurology
Imaging techniques
endoscopes
Light propagation
animal models
fibers
Fibers
cortexes
hair
footprints
neurons
Neurons
bundles
field of view
mice

Cite this

Turtaev, S., Leite, I. T., Altwegg-Boussac, T., Pakan, J. M. P., Rochefort, N. L., & Čižmár, T. (2018). High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging. Light: Science and Applications, 7, 1-8. [92]. https://doi.org/10.1038/s41377-018-0094-x
Turtaev, Sergey ; Leite, Ivo T. ; Altwegg-Boussac, Tristan ; Pakan, Janelle M. P. ; Rochefort, Nathalie L. ; Čižmár, Tomáš. / High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging. In: Light: Science and Applications. 2018 ; Vol. 7. pp. 1-8.
@article{cfee986506a2499ba7519209b80be9ed,
title = "High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging",
abstract = "Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 ± 0.04 µm across a 50-µm field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.",
author = "Sergey Turtaev and Leite, {Ivo T.} and Tristan Altwegg-Boussac and Pakan, {Janelle M. P.} and Rochefort, {Nathalie L.} and Tom{\'a}š Čižm{\'a}r",
note = "This work was funded by Marie Curie Actions of the European Union’s FP7 program (608144 to S.T. and I.T.L., IEF 624461 to J.P. and MC-CIG 631770 to N.R.); the European Regional Development Fund (ERDF: Center for Behavioral Brain Sciences to J.P. and CZ.02.1.01/0.0/0.0/15_003/0000476 to T.Č.); the European Research Council (ERC: 724530) to T.Č.; Th{\"u}ringer Ministerium f{\"u}r Wirtschaft, Wissenschaft und Digitale Gesellschaft, Th{\"u}ringer Aufbaubank and the Federal Ministry of Education and Research, Germany (BMBF) to S.T., I.T.L. and T.Č.; the Wellcome Trust, the Royal Society (Sir Henry Dale fellowship), the Shirley Foundation, the Patrick Wild Centre, the RS MacDonald Charitable Trust Seedcorn grant, and the Simons Initiative for the Developing Brain to N.R.; and the University of Dundee and Scottish Universities Physics Alliance (PaLS initiative) to T.Č.",
year = "2018",
month = "11",
day = "21",
doi = "10.1038/s41377-018-0094-x",
language = "English",
volume = "7",
pages = "1--8",
journal = "Light: Science and Applications",
issn = "2047-7538",
publisher = "Nature Publishing Group",

}

Turtaev, S, Leite, IT, Altwegg-Boussac, T, Pakan, JMP, Rochefort, NL & Čižmár, T 2018, 'High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging', Light: Science and Applications, vol. 7, 92, pp. 1-8. https://doi.org/10.1038/s41377-018-0094-x

High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging. / Turtaev, Sergey; Leite, Ivo T.; Altwegg-Boussac, Tristan; Pakan, Janelle M. P.; Rochefort, Nathalie L. (Lead / Corresponding author); Čižmár, Tomáš (Lead / Corresponding author).

In: Light: Science and Applications, Vol. 7, 92, 21.11.2018, p. 1-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

AU - Turtaev, Sergey

AU - Leite, Ivo T.

AU - Altwegg-Boussac, Tristan

AU - Pakan, Janelle M. P.

AU - Rochefort, Nathalie L.

AU - Čižmár, Tomáš

N1 - This work was funded by Marie Curie Actions of the European Union’s FP7 program (608144 to S.T. and I.T.L., IEF 624461 to J.P. and MC-CIG 631770 to N.R.); the European Regional Development Fund (ERDF: Center for Behavioral Brain Sciences to J.P. and CZ.02.1.01/0.0/0.0/15_003/0000476 to T.Č.); the European Research Council (ERC: 724530) to T.Č.; Thüringer Ministerium für Wirtschaft, Wissenschaft und Digitale Gesellschaft, Thüringer Aufbaubank and the Federal Ministry of Education and Research, Germany (BMBF) to S.T., I.T.L. and T.Č.; the Wellcome Trust, the Royal Society (Sir Henry Dale fellowship), the Shirley Foundation, the Patrick Wild Centre, the RS MacDonald Charitable Trust Seedcorn grant, and the Simons Initiative for the Developing Brain to N.R.; and the University of Dundee and Scottish Universities Physics Alliance (PaLS initiative) to T.Č.

PY - 2018/11/21

Y1 - 2018/11/21

N2 - Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 ± 0.04 µm across a 50-µm field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.

AB - Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 ± 0.04 µm across a 50-µm field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.

UR - http://www.scopus.com/inward/record.url?scp=85057042186&partnerID=8YFLogxK

U2 - 10.1038/s41377-018-0094-x

DO - 10.1038/s41377-018-0094-x

M3 - Article

VL - 7

SP - 1

EP - 8

JO - Light: Science and Applications

JF - Light: Science and Applications

SN - 2047-7538

M1 - 92

ER -