TY - JOUR
T1 - High-performance liquid chromatographic method for the separation and quantitative estimation of anti-parasitic melaminophenyl arsenical compounds
AU - Berger, Bradley J.
AU - Fairlamb, Alan H.
N1 - Funding Information:
Acknowledgements We acknowledge support from the IJNDPiWorld Bank/WHO Special Programmef or Researcha nd Training in Tropical Disease and the WellcomeT rust (A.H.F.) and the NATO ScienceF ellowshipP rogram( B.J .B.).
PY - 1994/5/1
Y1 - 1994/5/1
N2 - A sensitive high-performance liquid chromatographic method has been developed for the separation and quantitative estimation of melaminophenyl arsenical drugs used in the treatment of human and veterinary trypanosomiases. Five clinically relevant compounds (melarsoprol, melarsonyl potassium, cymelarsan, melarsen oxide, and sodium melarsen) can be separated with an octadecylsilane reverse-phase column and detected down to 10 pmol per injection. The chromatographic system utilizes a propanol gradient in water, with lithium camphor sulphonate as ion modifier, and detection by ultravioletabsorbance at 280 nm. Preliminary results indicate that the arsenical compounds can be extracted and concentrated from serum using octadecylsilane solid-phase extraction cartridges. This method represents the first specific and sensitive assay system for separating and quantitatively estimating this important class of antiparasitic agents.
AB - A sensitive high-performance liquid chromatographic method has been developed for the separation and quantitative estimation of melaminophenyl arsenical drugs used in the treatment of human and veterinary trypanosomiases. Five clinically relevant compounds (melarsoprol, melarsonyl potassium, cymelarsan, melarsen oxide, and sodium melarsen) can be separated with an octadecylsilane reverse-phase column and detected down to 10 pmol per injection. The chromatographic system utilizes a propanol gradient in water, with lithium camphor sulphonate as ion modifier, and detection by ultravioletabsorbance at 280 nm. Preliminary results indicate that the arsenical compounds can be extracted and concentrated from serum using octadecylsilane solid-phase extraction cartridges. This method represents the first specific and sensitive assay system for separating and quantitatively estimating this important class of antiparasitic agents.
UR - http://www.scopus.com/inward/record.url?scp=0028263714&partnerID=8YFLogxK
U2 - 10.1016/0035-9203(94)90117-1
DO - 10.1016/0035-9203(94)90117-1
M3 - Article
C2 - 7974690
AN - SCOPUS:0028263714
SN - 0035-9203
VL - 88
SP - 357
EP - 359
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 3
ER -
