High-resolution 3D mapping of rhizosphere glycan patterning using molecular probes in a transparent soil system

Catherine Y. Jones (Lead / Corresponding author), Ilonka Engelhardt, Daniel Patko, Lionel Dupuy, Nicola Holden, William G. T. Willats (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
22 Downloads (Pure)

Abstract

Rhizospheres are microecological zones at the interface of roots and soils. Interactions between bacteria and roots are critical for maintaining plant and soil health but are difficult to study because of constraints inherent in working with underground systems. We have developed an in-situ rhizosphere imaging system based on transparent soils and molecular probes that can be imaged using confocal microscopy. We observed spatial patterning of polysaccharides along roots and on cells deposited into the rhizosphere and also co-localised fluorescently tagged soil bacteria. These studies provide insight into the complex glycan landscape of rhizospheres and suggest a means by which root / rhizobacteria interactions can be non-disruptively studied.

Original languageEnglish
Article number100059
Number of pages4
JournalCell Surface
Volume7
Early online date16 Jul 2021
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Rhizobacteria
  • Transparent Soil
  • Rhizosphere
  • Polysaccharide
  • Fluorescence Microscopy

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