High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

D. Bulgarelli; N. C. Collins; G. Tacconi; E. Dellaglio; R. Brueggeman; A. Kleinhofs; A. M. Stanca; G. Valè

doi:10.1007/s00122-003-1557-9

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

D. Bulgarelli, N. C. Collins, G. Tacconi, E. Dellaglio, R. Brueggeman, A. Kleinhofs, A. M. Stanca, G. Valè

Plant Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F₂ population of 1,400 plants (ThibautRdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

Original language	English
Pages (from-to)	1401-1408
Number of pages	8
Journal	Theoretical and Applied Genetics
Volume	108
Issue number	7
DOIs	https://doi.org/10.1007/s00122-003-1557-9
Publication status	Published - May 2004

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

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title = "High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley",

abstract = "The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.",

author = "D. Bulgarelli and Collins, {N. C.} and G. Tacconi and E. Dellaglio and R. Brueggeman and A. Kleinhofs and Stanca, {A. M.} and G. Val{\`e}",

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AU - Bulgarelli, D.

AU - Collins, N. C.

AU - Tacconi, G.

AU - Dellaglio, E.

AU - Brueggeman, R.

AU - Kleinhofs, A.

AU - Stanca, A. M.

AU - Valè, G.

PY - 2004/5

Y1 - 2004/5

N2 - The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

AB - The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

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JO - Theoretical and Applied Genetics

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ER -

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

Abstract

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