High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

D. Bulgarelli, N. C. Collins, G. Tacconi, E. Dellaglio, R. Brueggeman, A. Kleinhofs, A. M. Stanca, G. Valè

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

Original languageEnglish
Pages (from-to)1401-1408
Number of pages8
JournalTheoretical and Applied Genetics
Volume108
Issue number7
DOIs
Publication statusPublished - May 2004

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Hordeum
chromosome mapping
barley
rice
Genes
leaves
genes
Pyrenophora graminea
chromosomes
Synteny
Dominant Genes
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 1
dominant genes
Sequence Analysis
Seeds
Oryza
pathogens
seeds
Population

Cite this

Bulgarelli, D., Collins, N. C., Tacconi, G., Dellaglio, E., Brueggeman, R., Kleinhofs, A., ... Valè, G. (2004). High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. Theoretical and Applied Genetics, 108(7), 1401-1408. https://doi.org/10.1007/s00122-003-1557-9
Bulgarelli, D. ; Collins, N. C. ; Tacconi, G. ; Dellaglio, E. ; Brueggeman, R. ; Kleinhofs, A. ; Stanca, A. M. ; Valè, G. / High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. In: Theoretical and Applied Genetics. 2004 ; Vol. 108, No. 7. pp. 1401-1408.
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Bulgarelli, D, Collins, NC, Tacconi, G, Dellaglio, E, Brueggeman, R, Kleinhofs, A, Stanca, AM & Valè, G 2004, 'High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley', Theoretical and Applied Genetics, vol. 108, no. 7, pp. 1401-1408. https://doi.org/10.1007/s00122-003-1557-9

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. / Bulgarelli, D.; Collins, N. C.; Tacconi, G.; Dellaglio, E.; Brueggeman, R.; Kleinhofs, A.; Stanca, A. M.; Valè, G.

In: Theoretical and Applied Genetics, Vol. 108, No. 7, 05.2004, p. 1401-1408.

Research output: Contribution to journalArticle

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AU - Bulgarelli, D.

AU - Collins, N. C.

AU - Tacconi, G.

AU - Dellaglio, E.

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