High-resolution genomic profiling of human papillomavirus-associated vulval neoplasia

K. J. Purdie; C. A. Harwood; K. Gibbon; T. Chaplin; B. D. Young; J. B. Cazier; N. Singh; I. M.  Leigh; C. M.  Proby

doi:10.1038/sj.bjc.6605589

High-resolution genomic profiling of human papillomavirus-associated vulval neoplasia

K. J. Purdie (Lead / Corresponding author), C. A. Harwood, K. Gibbon, T. Chaplin, B. D. Young, J. B. Cazier, N. Singh, I. M. Leigh, C. M. Proby

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

BACKGROUND: The incidence of human papillomavirus-associated vulval neoplasia is increasing worldwide; yet the associated genetic changes remain poorly understood.

METHODS: We have used single-nucleotide polymorphism microarray analysis to perform the first high-resolution investigation of genome-wide allelic imbalance in vulval neoplasia. Our sample series comprised 21 high-grade vulval intraepithelial neoplasia and 6 vulval squamous cell carcinomas, with paired non-lesional samples used to adjust for normal copy number variation.

RESULTS: Overall the most common recurrent aberrations were gains at 1p and 20, with the most frequent deletions observed at 2q, 3p and 10. Copy-neutral loss of heterozygosity at 6p was a recurrent event in vulval intraepithelial neoplasia. The pattern of genetic alterations differed from the characteristic changes we previously identified in cutaneous squamous cell carcinomas. Vulval neoplasia samples did not exhibit gain at 5p, a frequent recurrent aberration in a series of cervical tumours analysed elsewhere using an identical protocol.

CONCLUSION: This series of 27 vulval samples comprises the largest systematic genome-wide analysis of vulval neoplasia performed to date. Despite shared papillomavirus status and regional proximity, our data suggest that the frequency of certain genetic alterations may differ in vulval and cervical tumours. British Journal of Cancer (2010) 102, 1044-1051. doi:10.1038/sj.bjc.6605589 www.bjcancer.com (C) 2010 Cancer Research UK

Original language	English
Pages (from-to)	1044-1051
Number of pages	8
Journal	British Journal of Cancer
Volume	102
Issue number	6
DOIs	https://doi.org/10.1038/sj.bjc.6605589
Publication status	Published - 16 Mar 2010

Keywords

SNP array
vulva
VIN
VSCC
human papillomavirus
chromosome aberration
SQUAMOUS-CELL CARCINOMA
INTRAEPITHELIAL NEOPLASIA
CERVICAL-CANCER
HYBRIDIZATION ANALYSIS
GENETIC ALTERATIONS
COPY NUMBER
CHROMOSOMAL-ABERRATIONS
HPV
GAIN
REVEALS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/sj.bjc.6605589

Cite this

@article{210db5bef5b34730818d3e06f8fcbd1e,

title = "High-resolution genomic profiling of human papillomavirus-associated vulval neoplasia",

abstract = "BACKGROUND: The incidence of human papillomavirus-associated vulval neoplasia is increasing worldwide; yet the associated genetic changes remain poorly understood.METHODS: We have used single-nucleotide polymorphism microarray analysis to perform the first high-resolution investigation of genome-wide allelic imbalance in vulval neoplasia. Our sample series comprised 21 high-grade vulval intraepithelial neoplasia and 6 vulval squamous cell carcinomas, with paired non-lesional samples used to adjust for normal copy number variation.RESULTS: Overall the most common recurrent aberrations were gains at 1p and 20, with the most frequent deletions observed at 2q, 3p and 10. Copy-neutral loss of heterozygosity at 6p was a recurrent event in vulval intraepithelial neoplasia. The pattern of genetic alterations differed from the characteristic changes we previously identified in cutaneous squamous cell carcinomas. Vulval neoplasia samples did not exhibit gain at 5p, a frequent recurrent aberration in a series of cervical tumours analysed elsewhere using an identical protocol.CONCLUSION: This series of 27 vulval samples comprises the largest systematic genome-wide analysis of vulval neoplasia performed to date. Despite shared papillomavirus status and regional proximity, our data suggest that the frequency of certain genetic alterations may differ in vulval and cervical tumours. British Journal of Cancer (2010) 102, 1044-1051. doi:10.1038/sj.bjc.6605589 www.bjcancer.com (C) 2010 Cancer Research UK",

keywords = "SNP array, vulva, VIN, VSCC, human papillomavirus, chromosome aberration, SQUAMOUS-CELL CARCINOMA, INTRAEPITHELIAL NEOPLASIA, CERVICAL-CANCER, HYBRIDIZATION ANALYSIS, GENETIC ALTERATIONS, COPY NUMBER, CHROMOSOMAL-ABERRATIONS, HPV, GAIN, REVEALS",

author = "Purdie, {K. J.} and Harwood, {C. A.} and K. Gibbon and T. Chaplin and Young, {B. D.} and Cazier, {J. B.} and N. Singh and Leigh, {I. M.} and Proby, {C. M.}",

year = "2010",

month = mar,

day = "16",

doi = "10.1038/sj.bjc.6605589",

language = "English",

volume = "102",

pages = "1044--1051",

journal = "British Journal of Cancer",

issn = "0007-0920",

publisher = "Springer Nature",

number = "6",

}

TY - JOUR

T1 - High-resolution genomic profiling of human papillomavirus-associated vulval neoplasia

AU - Purdie, K. J.

AU - Harwood, C. A.

AU - Gibbon, K.

AU - Chaplin, T.

AU - Young, B. D.

AU - Cazier, J. B.

AU - Singh, N.

AU - Leigh, I. M.

AU - Proby, C. M.

PY - 2010/3/16

Y1 - 2010/3/16

N2 - BACKGROUND: The incidence of human papillomavirus-associated vulval neoplasia is increasing worldwide; yet the associated genetic changes remain poorly understood.METHODS: We have used single-nucleotide polymorphism microarray analysis to perform the first high-resolution investigation of genome-wide allelic imbalance in vulval neoplasia. Our sample series comprised 21 high-grade vulval intraepithelial neoplasia and 6 vulval squamous cell carcinomas, with paired non-lesional samples used to adjust for normal copy number variation.RESULTS: Overall the most common recurrent aberrations were gains at 1p and 20, with the most frequent deletions observed at 2q, 3p and 10. Copy-neutral loss of heterozygosity at 6p was a recurrent event in vulval intraepithelial neoplasia. The pattern of genetic alterations differed from the characteristic changes we previously identified in cutaneous squamous cell carcinomas. Vulval neoplasia samples did not exhibit gain at 5p, a frequent recurrent aberration in a series of cervical tumours analysed elsewhere using an identical protocol.CONCLUSION: This series of 27 vulval samples comprises the largest systematic genome-wide analysis of vulval neoplasia performed to date. Despite shared papillomavirus status and regional proximity, our data suggest that the frequency of certain genetic alterations may differ in vulval and cervical tumours. British Journal of Cancer (2010) 102, 1044-1051. doi:10.1038/sj.bjc.6605589 www.bjcancer.com (C) 2010 Cancer Research UK

AB - BACKGROUND: The incidence of human papillomavirus-associated vulval neoplasia is increasing worldwide; yet the associated genetic changes remain poorly understood.METHODS: We have used single-nucleotide polymorphism microarray analysis to perform the first high-resolution investigation of genome-wide allelic imbalance in vulval neoplasia. Our sample series comprised 21 high-grade vulval intraepithelial neoplasia and 6 vulval squamous cell carcinomas, with paired non-lesional samples used to adjust for normal copy number variation.RESULTS: Overall the most common recurrent aberrations were gains at 1p and 20, with the most frequent deletions observed at 2q, 3p and 10. Copy-neutral loss of heterozygosity at 6p was a recurrent event in vulval intraepithelial neoplasia. The pattern of genetic alterations differed from the characteristic changes we previously identified in cutaneous squamous cell carcinomas. Vulval neoplasia samples did not exhibit gain at 5p, a frequent recurrent aberration in a series of cervical tumours analysed elsewhere using an identical protocol.CONCLUSION: This series of 27 vulval samples comprises the largest systematic genome-wide analysis of vulval neoplasia performed to date. Despite shared papillomavirus status and regional proximity, our data suggest that the frequency of certain genetic alterations may differ in vulval and cervical tumours. British Journal of Cancer (2010) 102, 1044-1051. doi:10.1038/sj.bjc.6605589 www.bjcancer.com (C) 2010 Cancer Research UK

KW - SNP array

KW - vulva

KW - VIN

KW - VSCC

KW - human papillomavirus

KW - chromosome aberration

KW - SQUAMOUS-CELL CARCINOMA

KW - INTRAEPITHELIAL NEOPLASIA

KW - CERVICAL-CANCER

KW - HYBRIDIZATION ANALYSIS

KW - GENETIC ALTERATIONS

KW - COPY NUMBER

KW - CHROMOSOMAL-ABERRATIONS

KW - HPV

KW - GAIN

KW - REVEALS

UR - http://www.scopus.com/inward/record.url?scp=77949517543&partnerID=8YFLogxK

U2 - 10.1038/sj.bjc.6605589

DO - 10.1038/sj.bjc.6605589

M3 - Article

SN - 0007-0920

VL - 102

SP - 1044

EP - 1051

JO - British Journal of Cancer

JF - British Journal of Cancer

IS - 6

ER -

High-resolution genomic profiling of human papillomavirus-associated vulval neoplasia

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this