High Resolution Microultrasound (μUS) Investigation of the Gastrointestinal (GI) Tract

Thineskrishna Anbarasan, Christine E. M. Démoré, Holly Lay, Mohammed R. S. Sunoqrot, Romans Poltarjonoks, Sandy Cochran, Benjamin F. Cox (Lead / Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

    2 Citations (Scopus)

    Abstract

    High resolution, microultrasound (μUS) scanning of the gastrointestinal (GI) tract has potential as an important transmural imaging modality to aid in diagnosis. Operating at higher frequencies than conventional clinical ultrasound instruments, μUS is capable of providing scanned images of the GI tract with higher resolution. To investigate the use of μUS for this application, a phantom which is cost effective, within ethical guidelines and, most importantly, similar in histology to the human GI tract is necessary. Therefore, a phantom utilizing porcine small bowel tissue has been developed for custom assembled μUS scanning systems. Two such systems, a stepping scanner and a continuous sweep scanner were utilized to repeatedly scan regions of prepared samples of porcine small bowel tissue. The porcine small bowel tissue phantom was perfused with degassed phosphate buffer saline (dPBS) solution through a cannula inserted in its mesenteric vessel to simulate in vivo conditions and achieve better μUS mucosal characterization. The μUS system scans a transducer across the tissue phantom to acquire RF echo data, which is then processed using MATLAB. A B-scan reconstruction produces 2D images with relative echo strength mapped to a color map of the user's choice. The phantom developed also allows for modifications such as the insertion of fiducial markers to detect tissue change over time and simultaneous perfusion and scanning, providing a platform for more detailed research and investigation into μUS scanning of the GI tract.

    Original languageEnglish
    Title of host publicationBiosensors and Biodetection
    Subtitle of host publicationMethods and Protocols, Volume 2: Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors
    EditorsBen Prickril , Avraham Rasooly
    Place of PublicationNew York
    PublisherSpringer
    Pages541-561
    Number of pages21
    ISBN (Electronic)9781493969111
    ISBN (Print)9781493969104
    DOIs
    Publication statusPublished - 16 Mar 2017

    Publication series

    NameMethods in molecular biology
    PublisherSpringer
    Volume1572
    ISSN (Print)1064-3745

    Keywords

    • Microultrasound
    • High resolution ultrasound
    • Gastrointestinal tract
    • Porcine small bowel tissue
    • Phantom development

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